铜绿假单胞菌中OXA基因的分布与耐药性研究

Distribution of OXA-10 gene in Pseudomonas aeruginosa and drug resistance

目的调查医院铜绿假单胞菌临床分离株苯唑西林酶(OXA)基因的分布以及与铜绿假单胞菌耐药谱的关系,并初步探讨其在分子流行病学分析中的作用。方法收集临床2010年1-9月分离的铜绿假单胞菌75株进行OXA基因检测,采用PCR法检测OXA和整合酶基因,通过DNA直接测序确定OXA;统计铜绿假单胞菌的药敏结果,并分析基因型和耐药性之间的关。结果根据PCR产物片段大小及测序分析,75株铜绿假单胞菌中共有OXA-10基因阳性菌株35株,阳性率为46.7%;含有OXA-10基因的铜绿假单胞菌菌株整合酶基因阳性率为71.4%。结论 OXA-10基因广泛存在于铜绿假单胞菌临床分离株中,并可能通过整合子和其他基因同时定位于传递性质粒上造成传播,导致细菌多药耐药。

OBJECTIVE To investigate the prevalence of OXA-10 gene and to analyze their effect on the drug resistance in multi drug-resistant strains of Pseudomonas aeruginosa（PAE）.METHODS A total of 75 isolates of PAE were isolated from clinical specimens in our hospital from Jan to Sep 2010.OXA-10 and interase gene（int） was determined by PCR.All PCR products of OXA-10 were sequenced for determination of OXA-10.RESULTS Totally 35 isolates of 75 strains were OXA-10 positive and the positive rates of it in the 35 isolates carrying OXA-10 were 25（71.4%）,all the 35 isolates carrying OXA-10 were MDR PA.CONCLUSION The study shows that OXA-10 are widely detected,and also prevalent in PAE which can lead to resistant to almost all antibiotic at a high level and OXA-10 gene and integrase gene may coexist in transmissible plasmid by integron.