假丝酵母菌属分离鉴定与耐药性机制的分析

Isolation and identification of Candida and drug resistance mechanism

目的分析假丝酵母菌属临床感染病原菌的种类分布及药物敏感性。方法采用VITEK-32半自动细菌检测分析仪,YBC鉴定卡片进行鉴定和药敏试验。结果所分离的1003株假丝酵母菌属中,白色假丝酵母菌检出率占第1位,占58.9%,其次为热带假丝酵母菌占23.4%;假丝酵母菌属在痰液中检出率最高,占49.2%,其次为尿液,占24.6%;热带假丝酵母菌对伏立康唑敏感率仅为0.9%,对氟康唑、5-氟胞嘧啶、伊曲康唑有不同程度的耐药;光滑假丝酵母菌对氟康唑、伊曲康唑的敏感率也只达到79.8%。结论假丝酵母菌属感染种类增多,耐药性增加,对易感患者应重视监测,加强其免疫力,预防假丝酵母菌属感染。

OBJECTIVE To analyze the types distribution and drug sensitivity of Candida caused to nosocomial infections.METHODS Semiautomatic VITEK-32 analyzer detecting bacteria and YBC identify cards were used to determine and conduct drug sensitivity test.RESULTS In the 1003 strains Candida separated,Candida albicans was the most common pathogen,accounting for 58.9%,the second detectable pathogen was C.tropicalis,accounting for 23.4%.the detected rate of Candida in sputum specimens was highest 49.2%,in urine was the second 24.6%.The sensitive rate of C.tropicalis to voriconazole was 0.9%,and different levels drug tolerance to fluconazole,5-fluorocytosine,itraconazole;The sensitive rate of C.glabrata to fluconazole and itraconazole was 79.8%.CONCLUSION The types of Candida resulting in nosocomial infection and the drug resistance are increased,so susceptible patients should be focused on and surveyed,whose immunity should be enhanced to prevent nosocomial infections caused by Candida.