LIT对小鼠抗弓形虫感染的保护作用

THE PROTECTIVE EFFECT AGAINST TOXOPLASMA INFECTION IN MICE IMMUNIZED WITH LASER\|IRRADIATED TOXOPLASMA TACHYZOITES\+*

目的： 探讨激光照射弓形虫速殖子（ＬＩＴ） 的免疫保护力及其诱导的免疫应答水平。方法： 应用４．５ ＷＬＩＴ免疫ＩＣＲ小鼠，并用活体弓形虫速殖子攻击感染，观察ＬＩＴ抗虫效果。分别用间接免疫荧光法和ＥＬＩＳＡ法测定Ｔ淋巴细胞ＣＤ４＋ ／ＣＤ８＋ 亚群及特异性ＩｇＧ抗体。结果： ＬＩＴ免疫的小鼠具有抗急性弓形虫感染的能力， 能延长小鼠生存时间， 并能导致机体ＣＤ４＋ 、ＣＤ４＋ ／ＣＤ８＋ 显著升高及特异性ＩｇＧ抗体产生。结论： ４．５ Ｗ ＬＩＴ经腹腔免疫接种小鼠， 能诱导部分的保护性免疫力，

AIM: To observe the level of immune responses and protective immunity in mice induced by laser\|irradiated Toxoplasma tachyzoites (LIT).METHODS: ICR mice were immunized with LIT. After one month, the mice were challenged with live Toxoplasma tachyzoites to observe the effect of LIT. Indirect immunofluorescence assay and ELISA were used to detect CD4\++/CD8\++ lymphocyte subpopulation and IgG antibody. RESULTS: Immunization with LIT could partially protect the mice from Toxoplasma infection, prolong the survival time, enhance the CD4\++/CD8\++ ratio and produce specific IgG antibody. CONCLUSION: Intraperitoneal injection with LIT can induce partial protective immunity and specifically elicit ICR mice to generate humoral and cell\|mediated immune responses.\;