恶性疟原虫青蒿琥酯敏感株与抗性株对氯喹及氨酚喹敏感性的比较

COMPARISON OF SENSITIVITY OF ARTESUNATE\|SENSITIVE AND ARTESUNATE\|RESISTANT PLASMODIUM FALCIPARUM TO CHLOROQUINE AND AMODIAQUINE\+*

目的： 了解恶性疟原虫抗青蒿琥酯株对氯喹及氨酚喹 （阿莫地喹） 有无交叉抗性及其与青蒿琥酯联合使用是否有增效作用。方法： 用Ｒｉｅｃｋｍ ａｎｎ 体外微量法比较青蒿琥酯敏感株与抗性株恶性疟原虫对氯喹和氨酚喹的敏感性。结果： 氯喹对青蒿琥酯敏感株与抗性株原虫的ＩＤ５０ 分别为９０．９ 及１１２．０ ｎｍ ｏｌ／Ｌ， ＩＤ９５ 均为３２０．０ｎｍ ｏｌ／Ｌ； 氨酚喹ＩＤ５０ 分别为５０．９ 及１３３．５ ｎｍ ｏｌ／Ｌ， ＩＤ９５ 均为３２０．０ ｎｍ ｏｌ／Ｌ。在联合用药组中， 青蒿琥酯及氯喹的完全抑制浓度（ＩＤ９５） 分别为３．２ 及２０．０ ｎｍ ｏｌ／Ｌ，为单用组的１／１２５ 和１／１６；青蒿琥酯及氨酚喹的ＩＤ９５ 分别为３．２ 及５．０ ｎｍ ｏｌ／Ｌ， 为单用组的１／１２５ 和１／６４。结论： 抗青蒿琥酯恶性疟原虫对氯喹、氨酚喹无明显交叉抗性，青蒿琥酯与这２ 种药物联用在体外测试有明显增效作用。

AIM: To explore the in vitro sensitivity of artesunate\|sensitive and \|resistant Plasmodium falciparum to chloroquine and amodiaquine and to observe the effect of artesunate combined with chloroquine and artesunate combined with amodiaquine on artesunate\|resistant P.falciparum. METHODS: The sensitivity of the artesunate\|sensitive and \|resistant P.falciparum to chloroquine and amodiaquine and their combination with artesunate was compared by using Rieckmann′s in vitro micro\|technique.RESULTS: The ID\-\{50\} and ID\-\{95\} values of chloroquine, amodiaquine and artesunete were 90\^9, 50\^9, 9\^6 nmol/L and 320\^0、320\^0, 40\^0 nmol/L to the artesunate\|sensitive P.falciparum respectively and were 112\^0, 133\^5, 85\^1 nmol/L and 320\^0, 320\^0, 400\^0 nmol/L to artesunate\|resistant P.falciparum, respectively. When artesunate was combined with chloroquine, the ID\-\{50\} values of the 2 drugs were 3\^2 and 20\^0 nmol/L to the artesunate\|resistant P.falciparum. When artesunate was combined with amodiaquine, the ID\-\{95\} values of the 2 drugs were 3\^2 and 5\^0 nmol/L to the artesunate\|resistant P.falciparum, respectively. CONCLUSION: The artesunate\|resistant P.falciparum has no cross resistance to chloroquine and amodiaquine, however, artesunate combined with chloroquine or amodiaquine exhibit an apparent synergistic effect in vitro.\;