急性肺损伤大鼠肺组织白细胞介素-13表达的初步研究

A preliminary study of IL-13 expression in rat lung with acute lung injury

目的： 动态观察急性肺损伤（ ＡＬＩ） 大鼠肺组织白细胞介素－ １３（ＩＬ－ １３） ｍ ＲＮＡ 表达的变化，初步探讨ＩＬ－ １３ ｍ ＲＮＡ 的变化与ＡＬＩ 发生、发展的关系。方法： 在脂多糖诱发的ＡＬＩ 大鼠模型，采用ＲＴ－ ＰＣＲ 方法，检测肺组织ＩＬ－ １３ ｍ ＲＮＡ 的表达。结果：正常肺组织表达一定水平的ＩＬ－ １３ ｍ ＲＮＡ；在ＡＬＩ 大鼠肺组织，ＩＬ－ １３ ｍ ＲＮＡ 表达呈短暂性上调，随即逐渐下调。结论： 正常肺组织可表达一定水平的ＩＬ－ １３ ｍ ＲＮＡ；在ＡＬＩ肺组织，ＩＬ－ １３ ｍ ＲＮＡ 表达逐渐下调，提示局部抗炎作用减弱，可能引起抗炎和致炎作用失衡，从而促进急性呼吸窘迫综合征的发生。

AIM:To explore relationship between interleukin-13(IL-13) mRNA expression and development of acute lung injury (ALI) changes of IL-13 mRNA in rat lung with ALI were observed. METHODS:Using semi-quantitative reverse-transcription polymerase chain reaction IL-13 mRNA in rat lung with ALI induced by lipopolysaccharide(LPS)were measured . RESULTS:Normal rat lung expressed IL-13 mRNA in some extent; expression of IL-13 mRNA increased temporarily and decreased gradually in lung after rats were injuried by LPS .CONCLUSION:Normal lung can express IL-13 mRNA .Down-regulation of IL-13 mRNA in lung with ALI suggested anti-inflammatory capacity was reduced in lung ,which may cause unbalance between anti-inflammatory and pre-inflammatory force which contribute to the development of acute respiratory distress syndrome.