摘要
目的探讨齐墩果酸对阿霉素诱导的培养人皮肤角质形成细胞凋亡的影响。方法用MTT法通过比色分析测定吸光度(A)值,检测不同剂量齐墩果酸和阿霉素给药对角质形成细胞增殖的影响,用流式细胞仪检测细胞凋亡。结果阿霉素抑制体外培养的人皮肤角质形成细胞增殖,先给予齐墩果酸处理细胞,然后给予阿霉素,齐墩果酸升高A值(P<0.05),降低细胞凋亡率(P<0.05)。结论先行给予适当剂量齐墩果酸处理细胞,能一定程度减轻阿霉素对细胞的损伤,表现出对阿霉素致凋亡的保护作用。
Objective To investigate the effect of oleanolic acid（OA） on adriamycin induced apoptosis of cultured human skin keratinocytes in vitro.Methods Human skin keratinocytes were cultured with different concentrations of oleanolic acid and adriamycin.Absorbance A was detected by colorimetric assay of MTT.The optical density（OD） values were showed on a scanning multiwell spectrophotometer.Apoptosis rate of human skin keratinocytes was detected by flow cytometry.Results We found that the proliferation of human skin keratinocytes was inhibited by adriamycin.If cells were pre-incubated with oleanolic acid,OD value was significantly higher and apoptosis induced by adriamycin was suppressed（P0.05）.Conclusion This study suggests that oleanolic acid could partially inhibit adriamycin induced apoptosis of cultured human skin keratinocytes.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2011年第2期93-95,共3页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
基金
江苏省中医药局中医药科研项目(H05103)
