肝细胞生长因子对大鼠内侧副韧带损伤后愈合的影响

Effects of hepatocyte growth factor on the properties of the healing medial collateral ligament

[目的]观察肝细胞生长因子对大鼠内侧副韧带(medial collateral ligament,MCL)损伤愈合后组织学及生物力学特性恢复的作用。[方法]成年雄性SD大鼠46只,随机分为实验组和对照组,每组23只。制备右膝关节MCL断裂模型,实验组每只大鼠在伤口局部注入5μg肝细胞生长因子,然后以5-0可吸收线褥式缝合;对照组注入等量生理盐水后缝合伤口。至术后第4周,无损伤情况下将实验组及对照组愈合处韧带取下,进行组织学检查、电镜检查及生物力学特性检查。[结果]HE染色见实验组胶原纤维排列较整齐,而对照组胶原纤维排列紊乱。电镜观察见实验组胶原纤维直径较对照组明显增粗,差异有统计学意义(P<0.05)。实验组愈合的MCL极限负荷和刚度均大于对照组,差异有显著意义(P<0.01)。[结论]肝细胞生长因子可以在组织学及生物力学特性方面促进内侧副韧带损伤愈合。本实验可以为内侧副韧带损伤后优质愈合提供一种新方法及理论依据。

[Objective] To investigate the effect of hepatocyte growth factor on the histological and biomechanical properties of the healing medial collateral ligament（MCL）. [Methods]Forty-six rats were equally randomly divided into 2 groups： the experimental group and the control group.MCL of all the rats were ruptured to establish the wound models.In the treatment group,5 μg of HGF was locally injected in the wound of each rat and then the wound was sutured.The control group received sodium chloride during the same period.Four weeks after surgery,each repaired site was dissected out for histological evaluation and biomechanical test. [Results]In control rats,the collagen fibers were not evenly spaced.The fiber bundles within granulation tissue from the HGF-treated rats were evenly spaced.The mean fibril diameter of fibers from HGF-treated group was higher（P0.05） than that of the control group.In terms of the mechanical properties of the healing femur-medial collateral ligament-tibia complexes,the stiffness and ultimate force values of HGF-treated group were significantly higher than that of the control group（P0.01）. [Conclusion]With the application of HGF,histological properties and biomechanical properties of the healing ligament were improved significantly compared to control.These findings may provide a basis for the improving of repairing ligaments.