载脂蛋白E基因敲除鼠不同部位动脉粥样硬化病变病理形态学分析

Pathomorphology analysis of atherosclerotic lesion in different site of apolipoproteinE gene knock-out mice

目的通过建立载脂蛋白E基因敲除鼠动脉粥样硬化(atherosclerosis,AS)动物模型,分析其不同部位AS病变的病理形态学特征。方法用高脂高胆固醇饲料喂养载脂蛋白E基因敲除鼠,并行右颈总动脉套环术建立AS动物模型。连续石蜡切片,HE染色观察套环侧和未套环侧颈总动脉及主动脉粥样硬化病变,进行病变分型。结果未套环侧颈总动脉未见病变,套环侧颈总动脉粥样硬化病变明显,属于Ⅰ、Ⅱ型病变的共10例,Ⅲ、Ⅳ、Ⅴ型病变的共14例;主动脉无病变的共8例,属于Ⅰ、Ⅱ型病变的共10例,Ⅲ、Ⅳ、Ⅴ型病变的共6例。结论载脂蛋白E基因敲除鼠套环形成的AS病变严重且发生、发展迅速,主动脉自发形成的AS病变发展缓慢。

Purpose To analyze the pathomorphology characteristic of atherosclerotic lesion in different site by establishing animal model of atherosclerosis in apolipoproteinE gene knock-out mice.Methods ApolipoproteinE gene knock-out mice were fed a western-type diet and operated with perivascular right common carotid collar placement to establish animal model of atherosclerosis.Serial paraffin sections were routinely stained with hematoxylin and eosin to observe atherosclerotic lesion in common carotids and aorta.Results Atherosclerotic lesion in common carotid collar placement was most serious,but there was no atherosclerotic lesion in common carotid non-collar placement.There were ten cases belong to Ⅰ,Ⅱtype atherosclerotic lesion in common carotid collar placement and aorta,fourteen cases in common carotid collar placement but six cases in aorta belong to Ⅲ,Ⅳ,Ⅴ type atherosclerotic lesion.Conclusions Atherosclerotic lesion induced by collar placement in common carotid happens and develops quickly,but spontaneous formative atherosclerotic lesion in aorta grows slowly.