摘要
目的：研究高温对小鼠腹腔巨噬细胞HSP70及原癌基因C－fos表达的影响，探讨高温对机体组织细胞的作用机制，为临床肿瘤高温治疗提供理论依据。方法：将小鼠分为两组，实验组和对照组，采用完整细胞免疫组化及原位RNA杂交技术对HSO70及C－fos进行检测。结果：实验组HP70和C－fos表达分别为704，11±584．25和668．94±384．07，对照组HSP70和C－fos表达分别为175．87±1100．7和445．88±284．9，实验组与对照组相比，HSP70和C－fos表达均有显著差异，P＜0．001及P＜0．05。结论：高温对小鼠腹腔巨噬细胞HSP70及原癌基团C－fos表达均有明显地促进作用。
How hyperthermia affects rat macrophage expression of HSP70 and C - fos oncogene is studied, in order to detect the machanism of hyperthermia to tissue cells to provide the theoretical basis for clinic tumor hyperthermia therapy. Methods: The rats are divided into two groups: expremental and control groups, using immnunohistochemical and site hybridization to check exoression of HSP70 and C - fos oncogene. Results: In expremental groups expression of HSP70 and C - fos are 704, 11±584. 25and 668 .94 ± 384.07; while in control groups,expression of HSP70 and c - fos are 175. 87 ± 100.7 and 445. 88 ± 284.9, make a comparision between the two groups. there is great difference: P < 0.001 and P< 0. 05. Conclusion: Hyperthermia to rat macrophage expression of HSP70 and C - fos mcogone have significant effects.
出处
《开封医专学报》
1999年第3期9-10,18,共3页
Journal of Henan University(Medical Science)
基金
河南省教委自然科学基金!98310020
