γ-干扰素对姜黄素抑制HL-60细胞增殖的影响

Effects of concurrent use of rh IFN -γ on the anti proliferative capacity on HL-60 cells caused by curcumin

目的 :为研究在姜黄素作用下 ,基因重组干扰素γ(rh IFN γ)对白血病细胞杀伤能力的影响。方法 :选用HL 6 0细胞系 ,采用细胞培养、SABC法测定Brdu摄取率、流式细胞仪检测及末端TdT酶标技术。结果 :发现姜黄素抑制细胞增殖作用随浓度增加而逐渐增加 ,在 2 5 μmol/L浓度体外处理HL 6 0细胞 2 4小时后 ,增殖抑制率为 43 75 %± 2 0 0 % ,IFN γ使这种作用明显提高 ,增殖抑制率增至 80 %。进一步分析Brdu摄取率和DNA含量分布图时发现姜黄素使细胞阻滞于G1 /G0 和G2 /M期 ,同时出现亚 G1 峰 (凋亡峰 )。IFN γ与姜黄素联用后 ,DNA合成率进一步下降 ,与两药单用组的结果相比有明显的差异 (P <0 0 5 )。同时 ,亚 G1 峰值也增高 ,同步的末端TdT酶标技术也反映联用组的凋亡细胞数与亚 G1 峰值呈一致变化。结论 :IFN

Objective:To understand the influence of rh IFN γ on the ability of curcumin to kill HL 60 cells in vitro Methods:The myeloid leukemia cell line HL 60 was studied using cell culture,MoAbBrdu,flow cytometry and TUNEL method Results:The data showed curcumin inhibited proliferation of leukemia cells in a dose dependent manner When HL 60 cells were treated with 25 μmol/L curcumin for 24 hours,the proliferative inhibitory rate was 43 75%±2 00% This effect could be enhanced obviously by IFN γ,the combined proliferative inhibitory rate increased to over 80% The 5 Brdu incoraportion rate and the distribution of DNA content were analyzed The results indicated curcumin could arrest cells in the G 1/G 0 and G 2/M phase of cell cycle At the same time,the sub G 1 peak (apoptotic peak) appeared After IFN γcombined with curcumin,DNA synthesis rate decreased further It had significant difference with singal drug group (P< 0 05) Meanwhile,it companied with the enhancement of sub G 1 peak The percent of TUNEL positive cells was aslo increased Conclusion:IFN γcan enhance the antiproferative capacity of curcumin against HL 60 cells