内源性1,25(OH)_2D_3缺乏对哺乳期小鼠牙齿矿化和牙本质形成的影响

Effect of Endogenous 1,25(OH)_2D_3 Deficiency on Dental Mineralization and Dentin Formation on Breast-feeding

目的:研究内源性1,25(OH)2D3缺乏对哺乳期小鼠牙齿矿化和发育的影响。方法:利用X线检测、HE染色、组织化学、免疫组织化学以及real-time RT-PCR等方法检测2周龄同窝的WT和1α(OH)ase-/-小鼠下颌牙齿。结果:和2周龄WT小鼠相比,1α(OH)ase-/-小鼠下颌磨牙的X线透光率明显增加;1α(OH)ase-/-小鼠下颌第一磨牙及下颌切牙前期牙本质厚度与WT小鼠厚度有统计学差异。1α(OH)ase-/-小鼠下颌磨牙总胶原阳性面积与WT小鼠有统计学差异;1α(OH)ase-/-小鼠下颌第一磨牙牙本质I型胶原阳性面积与WT小鼠有统计学差异(P<0.05);OCN在1α(OH)ase-/-小鼠下颌第一磨牙的牙本质和牙髓相对阳性面积与WT小鼠相对阳性面积有统计学差异(P<0.01);Biglycan在1α(OH)ase-/-小鼠下颌第一磨牙的牙本质相对阳性面积与WT小鼠相对阳性面积有统计学差异(P<0.01);OCN与ALP在1α(OH)ase-/-小鼠下颌切牙牙髓细胞中mRNA的表达水平较WT小鼠有明显降低,有统计学差异(P<0.05)。结论:内源性的1,25(OH)2D3缺乏导致哺乳期小鼠牙齿的牙本质矿化不良和牙本质的形成减少。

Objective： To research the effect of endogenous 1, 25 （OH）2D3 deficiency on mineralization and the de- velopment of teeth on breast-- feeding mice. Methods.. X-- ray detection, HE staining, histochemistry, immunohis- tochemistry and real--time RT--PCR were used to detect the WT littermates and la（OH）ase-/- mice mandible teeth at 2 weeks aged. Results.. Compared with WT mice, la（OH）ase-/- mice mandibular molar X--ray light transmission rate was significantly increased, la（OH）ase-/- mice pre-mandibular first molar of dentin thickness was （10.79~1.14）mm, however WT mice was （7.89~0.86）ram, la（OH） ase-/- mice mandibular incisors pre- dentin thickness was （12.18_--4-1.37）mm, while, the thickness of WT mice was （7.84_±0.79）mm. There were sig- nificant difference between both Ia（OH） ase-/- mice with collagen--positive area of total mandibular molars was （0.19±0. 018）ram2 , but the WT mice was （0.25±0. 021） mm2 , which was significantly different, la（OH）ase-/- mouse mandibular first molar dentin type Icollagen--positive area was （0.24 A-0. 019）mm2, while WT mice was （0.18zk0. 018）ram2, which was significantly different. OCN in la（OH）ase-/- mouse mandibular first molar of dentin and pulp relatively positive area was （5.68-1-0.87）~, but the WT mice was （14.21 ~ 2.41）%. Biglycan in la（OH）ase-/- mouse mandibular first molar dentin was （41.09-4-5.89）%, a relatively positive area in the WT mice was （22.43-1-3.18）%. There was significant differences. OCN and ALP in1 a （OH） ase-/- mice incisor dental pulp cells in mRNA expression levels significantly decreased compared with WT mice, which was significant- ly different. Conclusion： Endogenous 1, 25（OH）z D3 deficiency resulted abnormal mineralization and dentin forma- tion of teeth on breast--feeding mice.