低强度超声联合原卟啉IX诱导人舌鳞癌SAS细胞凋亡作用的研究

Low-intensity Ultrasound Combined with Protoporphyrin IX Induced Apoptosis on Human Tongue Cancer Cell Line SAS

目的:研究低强度超声联合原卟啉IX对人舌鳞癌SAS细胞增殖抑制及诱导凋亡作用。方法:SAS细胞分成对照和实验组,台盼兰和四甲基偶氮唑盐(MTT)法检测细胞活性,流式细胞仪检测细胞凋亡,透射电镜观察超微结构改变。结果:连续波强度结合100g/L原卟啉IX作用60s对SAS细胞增殖有显著的抑制作用,呈时间依赖性。透射电镜观察到凋亡小体及线粒体等细胞器改变。结论:低强度超声激活原卟啉IX诱导SAS细胞凋亡的作用机制可能与线粒体凋亡途径相关。

Objective： To study the low--intensity ultrasound combined with protoporphyrin IX on human tongue cancer cell line SAS proliferation and apoptosis. Methods： SAS cells were divided into control and experimental group, Cell activity was detected by trypan blue and tetrazolium salt （MTT）, and cell apoptosis was analyzed by flow cytometry. Results： The proliferation of SAS cells was inhibited significantly by the continuous--wave strength Combined with 100ug/ml protoporphyrin IX for 60s. The growth inhibition effect was time--dependent. The chan- ges of apoptotic bodies and mitochondrion were observed by TEM. Conclusion： The mechanism that low intensity ultrasound induced apoptosis of SAS cells significantly by activating protoporphyrin IX may be associated with mito- chondria apoptotic pathway.