MnTBAP对鼠面神经元损伤后凋亡的抑制作用

The inhibitive effect of MnTBAP on apoptosis of facial neurons following transection of facial nerve

目的:探讨外源性药物锰卟啉(MnTBAP)对面神经元凋亡的抑制作用。方法:制作大鼠面神经切断伤模型,分别于术后1d、3d、7d、14d、28d和42d时,利用免疫组织化学技术检测面神经元cyto-c、caspase-9和caspase-3的表达;利用原位末端标记法(TUNEL法)检测面神经元的凋亡情况。采用SPSS13.0软件包进行组间t检验。结果:术后1d,cyto-c、caspase-9表达水平开始升高,7d时达到高峰;术后3d时,caspase-3表达水平开始升高,TUNEL阳性细胞增多,14d时达到高峰。各时间点表达水平MnTBAP侧较对照侧显著降低(P<0.05)。结论:MnTBAP对损伤的面神经元有明显的抗凋亡作用,是一种有效的神经保护剂。

PURPOSE： The study is to investigate the inhibitive effect of MnTBAP on apoptosis of facial neurons following transection of facial nerve. METHODS： The bilateral facial nerve were exposed and transected. At 1d,3d,7d, 14d,2gd and 42d after operation, the expression of eyto-e,caspase-9 and caspase-3 were detected by immunohistoehemistry. The apoptotie cells of facial neurons were observed by TUNEL staining. Statistical analysis was performed with SPSS 13.0 software package. RESULTS： At ld, the expression of cyto-c, easpase-9 increased after transection and achieved the peak at 7d. At 3d ,the expression of easpase-3 increased and TUNEL-positive cells were observed, they reached the peak at 14d. The positive level in MnTBAP side was significantly lower than that in control side at each time point. There was significant difference between the experimental group and the control group （P〈0.05）. CONCLUSION： MnTBAP can effectively inhibit apoptosis of facial neurons and has neuroproteetive effect on facial nerve after transection.Supported by Science Planning Project of Shenyang City（200340）.