慢性乙型肝炎患者血清MIF、IL-17及IL-10的检测及其临床意义

Serum macrophage migration inhibitory factors,IL-17 and IL-10 in chronic hepatitis B:clinical correlations

目的探讨巨噬细胞移动抑制因子(MIF)、白细胞介素(IL)-17及IL-10在慢性乙型肝炎(乙肝)发病中的可能作用及临床意义。方法选择48例慢性乙肝患者(HBeAg阳性与阴性各24例;HBV DNA阴性21例,HBV DNA阳性27例)作为试验组,24例健康人作为对照组,采用酶联免疫吸附试验(ELISA)测定两组血清中MIF、IL-17及IL-10的含量。结果试验组与对照组结果比较,MIF[(67.50±9.21)μg/Lvs(25.21±7.08)μg/L]和IL-17[(70.93±2.24)vs(26.78±1.58)μg/L]明显升高,IL-10[(192.88±20.74)vs(240.32±28.33)μg/L]明显下降,差异均有统计学意义(P<0.05)。HBeAg阴性与阳性组、HBV DNA阴性与阳性组中MIF、IL-17及IL-10的含量差异均无统计学意义。MIF、IL-17的含量与ALT呈正相关(r=0.693,P<0.01;r=0.897,P<0.001),而IL-10与ALT呈负相关(r=-0.285,P=0.037)。结论 MIF、IL-17及IL-10可能参与慢性乙肝的发病过程,具有重要的临床意义。

Objective To explore clinical correlations of serum macrophage migration inhibitory factors（MIF）,IL-17 and IL-10 in chronic hepatitis B.Methods We randomly selected 48 patients of chronic hepatitis B（HBeAg positive：24;HBV-DNA positive：27） and 24 healthy volunteers for the study.Serum concentrations of MIF,IL-17 and IL-10 were evaluated by the methods of enzyme-linked immunosorbent assay（ELISA）.Results Serum concentrations of MIF and IL-17 were significantly higher in chronic hepatitis B patients[（67.50±9.21）μg/L and（70.93±2.24）μg/L]than in healthy controls[（25.21±7.08）μg/L and（26.78±1.58）μg/L]（P〈0.05）,while serum concentration of IL-10 was significantly lower in chronic hepatitis B patients[（192.88±20.74）μg/L]than in healthy controls[（240.32±28.33）μg/L]（P〈0.05）.There were no differences in serum concentrations of MIF,IL-17 and IL-10 between patients with positive and negative HBeAg or HBV-DNA.Serum ALT levels were positively correlated with concentrations of MIF and IL-17（r=0.693,P〈0.01;r=0.897,P 0.001,respectively） and negatively correlated with concentration of IL-10（r=-0.285,P〈0.05）.Conclusions MIF,IL-17 and IL-10 may be involved in the pathogenesis of chronic hepatitis B and have clinical significance.