白介素27基因多态性与广西壮族系统性红斑狼疮的相关性研究

Association of interleuldn-27 gene polymorphisms with genetic susceptibility to systematic lupus ery-thematosus in Guangxi Zhuang population

目的探讨白介素27（IL-27）基因单核苷酸多态性与广西壮族系统性红斑狼疮（sLE）易感性之间的关系。方法以135例SLE患者和150例正常人对照者为研究对象，应用聚合酶链反应-限制性片段长度多态性和DNA测序的方法对IL-27基因-964A／G、2905T／G单核苷酸多态性进行基因分型。结果SLE组和正常人对照组中IL-27基因2905T／G多态性分布差异无统计学意义（r=1．63，P〉0．05），而IL-27基因-964A／G多态性的分布差异有统计学意义（矿=9．88，P〈0．01）。等位基因频率的相对风险分析发现，-964G等位基因携带者患SLE的风险是-964A等位基因的1．725倍（OR=1．725，95％a：1．227～2．425）。联合基因型分析发现，IL-27的-964G／2905G等位基因频率SLE组（10．7％）显著高于对照组（5．3％）（P〈0．01），-964G／2905G等位基因携带者显著增加了SLE的发病风险（OR=2．351．95％CI：1．228。4．501）。结论IL-27基因-964MG多态性与SLE的发病具有相关性，其中一964G等位基因可能是SLE的遗传易感基因。

Objective To investigate the association between the single nucleotide polymorphisms of interleukin-27 （IL-27） gene and susceptibility to systematic lupus erythematosus （SLE） in Guangxi Zhuang population. Methods In total, 135 patients with SLE and 150 age- and sex-matched human controls of Zhuang nationality were recruited in this study. PCR-restriction fragment length polymorphism （RFLP） analysis and DNA sequencing were performed to analyze the IL-27 gene -964 A/G and -2905 T/G polymorphisms. Results Significant differences were observed in the distribution of IL-27 gene -964 A/G polymorphism （X2 = 9.88, P 〈 0.01 ）. The relative risk for SLE in carriers of G allele at position 964 of IL-27 gene was 1.725 times that in carriers of A allele at this position （OR = 1.725,95% CI： 1.227 - 2.425）. A significant increase was observed in the frequency of 964G/2905G alleles of IL-27 gene in patients with SLE compared with the con- trols （10.7% vs. 5.3%, P 〈 0.01 ）, and the 964G/2905G alleles were associated with a significantly increased risk for SLE （OR = 2.351, 95% CI： 1.228 - 4.501）. Conclusions The IL-27 gene -964 A/G polymorphism is associated with the development of SLE, and the -964 G allele may increase the genetic susceptibility to SLE.