EB病毒转化淋巴母细胞LMP-1,LMP-2A及LMP-2B的表达

Expressions of LMP-1,LMP-2A,and LMP-2B in EBV-transformed lymphoblasts

目的了解EB病毒转化淋巴母细胞LMP-1,LMP-2A,LMP-2B基因的表达改变。方法采用实时定量PCR方法分别检测并比较同一献血员来源的正常人淋巴细胞与EBV转化淋巴母细胞前后LMP基因(LMP-1、LMP-2A、LMP-2B)的表达改变。采用Western bolt检测LMP-1蛋白表达。结果 LMP-1、LMP-2A、LMP-2B基因在转化淋巴母细胞比在正常人淋巴细胞的表达分别上调863倍、1 763倍、90 078倍。Western bolt检测LMP-1蛋白在转化淋巴母细胞中的表达比正常人淋巴细胞明显增强。结论在EBV转化淋巴母细胞过程中LMP-1、LMP-2A和LMP-2B表达均上调。

We aim to detect differential expressions of LMP genes（LMP-1,LMP-2A,LMP-2B） in normal human lymphocytes and EBV-transformed lymphoblasts in vitro.Firstly,expression levels of LMP genes（LMP-1,LMP-2A,LMP-2B） in the two kinds of cells were detected by using real-time PCR.Then LMP-1 protein expression was detected by Western bolt in the normal lymphocytes and EBV-transformed lymphoblasts.It was found that LMP-1 mRNA expression was 863 times up-regulated in the transformed lymphoblasts,as compared with that in the normal lymphocytes（P 0.01）.Consequently,the expression of LMP-1 protein in EBV-transformed lymphoblasts was higher than that in the normal lymphocytes.LMP-2A mRNA expression was 1763 times up-regulated in the transformed lymphoblasts as compared with,that in the normal lymphocytes（P 0.05）;LMP-2B mRNA expression was 90078 times up-regulated in the transformed lymphoblasts as compared with that in the normal lymphocytes（P 0.05）.All the result show that the expressions of LMP-1,LMP-2A and LMP-2B genes would be upward in EBV-transformed lymphoblasts.