乳腺癌组织NRF-1表达及其意义的探讨

Significance of expression of NRF-1 protein in breast carcinoma tissues

目的:探讨乳腺癌组织中核呼吸因子-1(NRF-1)蛋白表达与乳腺癌发生、发展及临床病理特征的关系。方法:采用免疫组化EnVision二步法,对211例乳腺癌组织和50例乳腺良性病变组织中NRF-1蛋白表达进行检测。结果:NRF-1蛋白表达定位于细胞核,着色呈棕黄色,乳腺癌中阳性表达(82.7%)低于乳腺良性病变组织阳性表达(100%),χ2=100.288,P=0.000;高分化乳腺癌NRF-1阳性率明显高于中、低分化乳腺癌(χ2=16.242,P=0.001;χ2=72.802,P=0.000),中分化乳腺癌也明显高于低分化乳腺癌,χ2=30.190,P=0.000。乳腺癌淋巴结转移患者NRF-1蛋白阳性表达率明显低于未转移者,χ2=12.025,P=0.007;TNM分期中I期NRF-1蛋白阳性表达率明显高于Ⅱ、Ⅲ期,χ2=12.025,P=0.007。结论:NRF-1蛋白的表达可能与乳腺癌的发生发展密切相关,可作为乳腺癌患者疾病进展监控和预后观测的指标,具有临床应用价值。

OBJECTIVE： To study the relationship between the expression of NRF-1 protein and carcinogenesis,invasion,metastasis of the breast carcinoma.METHODS： NRF-1 protein expression in 211 cases of breast carcinoma and 50 cases benign lesion of the mammary gland was examined by immunohistochemical method.RESULTS： The NRF-1 protein located in nucleus.The positive rate of NRF-1 protein expression was 82.7% in breast carcinoma,and that was 100% in benign lesion of the mammary gland（χ2=100.288,P=0.000）.Expression rates of NRF-1 protein in the well-differentiated of breast carcinoma were significantly than mid-differentiated and low-differentiated of breast carcinoma（χ2=16.242,P=0.001;χ2=72.802,P=0.000）,and mid-differentiated higher than low-differentiated of breast carcinoma（χ2=30.190,P=0.000）.Low or negative NRF-1 protein expression was associated with positive lymph node metastasis in breast carcinoma（χ2=12.025,P=0.007）.The positive rate of NRF-1 protein was significantly lower in TNM Ⅱ,Ⅲ stage than TNM I stage（χ2=12.025,P=0.007）.CONCLUSION： NRF-1 protein expression may be correlated to the occurrence and development of breast carcinoma,and can be a marker in the progression and prognosis of breast carcinoma.