非小细胞肺癌组织B7-H3和CD133表达及其临床意义的研究

Expression and clinical significance of B7-H3 and CD133 in non-small cell lung cancer tissues

目的:探讨共信号分子B7-H3在人非小细胞肺癌(NSCLC)细胞系和组织中的表达及其临床意义,以及同CD133的关系。方法:利用逆转录-聚合酶链反应、流式细胞术及蛋白质印迹技术检测B7-H3在肺癌细胞系中的表达;采用免疫组化SP法检测102例NSCLC、25例癌旁组织及24例肺部良性病变组织中B7-H3及CD133的表达,并采用Image-ProPlus软件测定每张切片的平均积分光密度。结果:无论是mRNA水平还是蛋白水平,5个肺癌细胞系中均见B7-H3表达;B7-H3和CD133在NSCLC组织中的表达高于良性病变组织及癌旁组织,P<0.001;B7-H3的诊断灵敏度68.63%,特异度91.49%,CD133的诊断灵敏度54.90%特异度97.87%;CD133表达与肺癌细胞分化程度相关,分化差者阳性率高于分化好者,P=0.02;Cox回归分析显示,B7-H3阳性、CD133阴性表达患者的总体生存期明显优于B7-H3阴性及CD133阳性表达者,P<0.001。结论:B7-H3在NSCLC组织中过度表达,可能是NSCLC诊断和治疗的重要靶点,CD133与肿瘤细胞分化程度有关,它的表达与B7-H3呈负相关;B7-H3阴性或CD133阳性是NSCLC独立的风险因子。

OBJECTIVE： To investigate the expression and clinical significance of co-signal molecule B7-H3 in cell lines and tissues of non-small cell lung cancer（NSCLC）,and the relationship between B7-H3 and CD133.METHODS：B7-H3 expression was evaluated by flow cytometry,RT-PCR and western blot in five NSCLC cell lines.Specimens from 102 participants were stained immunohistochemically by mouse anti-human B7-H3 and CD133 monoclonal antibody.Staining was quantified using Image-Pro Plus software,and integrated optical density was measured on each section.RESULTS： The constitutive expression of B7-H3 in all five cell lines was found.The expressions of B7-H3 and CD133 were higher than that in benign lesions and non-cancer tissues respectively（P0.001）.The sensitivity and the specificity of B7-H3 expression in NSCLC were 68.63% and 91.49% respectively,and those of CD133 were 54.90% and 97.87%.CD133-positive was related to tumor cell poor differentiation（P=0.02）.B7-H3 positive or CD133 negative contributed to survival of participants by COX regression analysis（P0.001）.CONCLUSIONS： B7-H3 is overexpressed in NSCLC tissues and may be an important target for diagnosis or therapy of the disease.CD133 is related to tumor cell differentiation degree,and the expression is negative correlated with B7-H3 expression.CD133 positive or B7-H3 negative is an independent risk factor of NSCLC patients.