中年人中环氧化物水解酶2基因遗传多态G860A与原发性高血压相关性的研究

Association of EPHX2 polymorphism G860A with hypertension in middle ages

目的探讨环氧化物水解酶（EPHX）2基因遗传多态G860A与中年人原发性高血压的相关性。方法在原发性高血压患者100人及年龄、性别匹配的正常对照300人中，采用real-timePCR技术（TAQMAN技术）分析EPHX2基因遗传多态G860A的基因型及等位基因频率分布，并采用多因素非条件logistic回归分析分析EPHX2G860A基因多态的人群高血压易感性。结果EPHX2基因遗传变异G860A基因型频率在高血压与对照中的分布差异有统计学意义（P=0．01）；携带GA变异基因型个体发生高血压的危险性是GG基因型携带者的1．98倍（adjustedOR=1．98；95％CI：1．19—3．51），携带GG变异基因型个体的危险性为2．84倍（adjusted OR=2．84；95％CI=1．01—6．11），且存在显著的等位基因剂量一效应关联（Ptrend=0．03）。结论EPHX2基因的860A变异基因型升高了我国中年人高血压的发病风险，遗传变异G860A可作为高血压人群易感性的生物标志。

Objective To investigate the association between the EPHX2 polymorphism G860A and the risk of hypertension in mild ages. Methods In a hospital based case-control study, one common polymorphism G860A in EPHX2 gene in a case-control study of 100 hypertension and 300 age- and sex frequency - matched disease-free controls were genotyped in a Southern Chinese population. SAS 9. 13 was used to analysis the polymorphism and hypertension risk. Results Genotype frequencies of EPHX2 G860A locus between the cases and the controls were significantly different （ P =0. 01 ）. Compared with the most common 860GG genotype, the 860GA heterozygote had an increased risk of hypertension （ adjusted OR = 1.98; 95% CI = 1.19 -3.51 ）, the AA homozygote had a further increased risk of hypertension （adjusted OR =2. 84; 95% CI = 1. 01 -6. 11 ）. There was a significant trend for an allele dose effect on risk of hypertension （ P trend = 0. 03）. Conclusions EPHX2 polymorphism G860A is associated with an increased risk of hypertension, and the 860A variant may be a marker for susceptibility to hypertension.