Snail mRNA及E—cadherin mRNA在乳腺癌中的表达及临床意义

The expression of Snail mRNA and E-cadherin mRNA in breast cancer and their clinical significance

目的探讨Snail mRNA及E—cadherin mRNA在乳腺浸润性导管癌中的表达及其临床意义。方法应用原位杂交技术测30例乳腺单纯性增生、30例乳腺导管内癌和70例乳腺浸润性导管癌组织中Snail mRNA及E—cadherin mRNA的表达。结果Snail mRNA及E—cadherin mRNA在乳腺单纯性增生、乳腺导管内癌和乳腺浸润性导管癌组织中的阳性率分别为23．3％、46．7％、81．4％和96．7％、66．7％、35．7％，差异有统计学意义（P〈0．01）；Snail mRNA及E-cadherin mRNA在同组间表达差异有统计学意义（P〈0．05）；Snail mRNA表达与年龄、肿瘤大小和组织学分级无关（P〉0．05），淋巴结转移与否Snail mRNA表达差异有统计学意义（P〈0．01）；E-cad mRNA表达与年龄、肿瘤大小无关（P〉0．05），淋巴结转移与否、不同组织学分级E-cad mRNA表达差异有统计学意义（P〈0．01）；Snail mRNA与E-cadherin mRNA的表达呈负相关（r=-0．56，P=0．00）。结论Snail mRNA过表达与E-cadherin mRNA低表达可能参与乳腺癌浸润转移的发生，并与淋巴结转移密切相关，因而检测两者的表达对判断乳腺癌预后、转移有重要价值。

Objective To investigate the expressions of Snail mRNA and E-cad mRNA in invasive ductal carcinoma and their clinical significance. Methods The expression of Snail mRNA and E-cad mRNA were detected on mammary gland hyperplasia （30cases）, intraductal breast carcinoma （30cases） and invasive ductal carcinoma （70cases）by in situ hybridization. Results The positive expression rate of Snail mRNA and E-cad mRNA in mammary gland hyperplasia, intraductal breast carcinoma and invasive ductal carcinoma were 23.3% ,46. 7% ,81.4% and 96. 7% ,66.7% ,35.7% ,respectively. Therewas significant difference among them（ P 〈 0. 01 ）. There was difference between Snail mRNA and E-cad mRNA in the same group（ P 〈0. 05）. Snail mRNA was not related to age, tumor size or histopathological grade （ P 〉 0. 05）, but it was related to lymphatic metastasis （ P 〈 0. 01 ）. E-cad mRNA was not related to age, tumor size（ P 〉 0. 05）, but it was related to lymphatic metastasis and histopathological grade（ P 〈 0. 01 ）. There were positive relationship between Snail mRNA and E-cad mRNA（ r = -0. 56, P =0. 00）. Conclusions The overexpression of Snail mRNA and low expression of E-cad mRNA were involved in the infiltration and metastasis of breast carcinoma, and they were related to lymphatic metastasis. Therefore, the test of the expression of them were valuable in predicting the prognostic and metastasis of breast carcinoma.