扛板归对肝纤维化大鼠肝组织缺氧诱导因子1α及血管内皮生长因子表达的影响

The effect of polygonum perfoliatum L on HIF-laand VEGF of hepatic tissue of hepatic fibrosis rats

目的研究中药扛板归对二甲基亚硝胺诱导的肝纤维化大鼠肝组织缺氧诱导因子1α（HIF-1α）及血管内皮生长因子（VEGF）蛋白表达的影响。方法72只SD大鼠，按随机数字表法分为六组，每组12只，用二甲基亚硝胺诱导大鼠肝纤维化模型，不同浓度扛板归干预后，肝脏常规制片并HE染色观察病理形态学改变，免疫组织化学方法检测各组大鼠肝组织HIF-1α及VEGF蛋白表达的情况。结果扛板归高剂量组中HIF-1α及VEGF蛋白表达情况从（-）～（3＋）分别为1只，9只，1只，0只和1只，8只，2只，0只、中剂量组大鼠肝组织HIF-1α及VEGF蛋白表达从（-）-（3＋）分别为1只，7只，2只，0只和1只，6只，3只，0只，与模型组比较均明显降低（P〈0．01）。结论扛板归具有显著的抗肝纤维化，其机制可能通过降低HIF—1α及VEGF蛋白的表达而调控细胞外基质的代谢有关。

Objective To assess the effect of polygonum perfoliatum L on the expression of Hypoxia inducible factor-1 （HIF-1α） and vascular endothelia growth factor （VEGF） in hepatic fibrosis rats induced by Dimethylnitrosamine （DMN） . Methods Seventy-two SD rats were randomly divided into six groups, after the model of HF rat induced by DMN and intervened by different concentrations of Polygonum perfoliatum L. Conventional flaking of liver and HE were used to observed histopathological change. Immunohistochemstry was used to detect HIF-1αand VEGF in hepatic tissues of hepatic fibrosis rats. Results The expression of HIF-1αand VEGF in hepatic fibrosis rats of the high dose of Polygonum perfoliatum L from （ - ） to （3 ＋ ） were one ,nine, one, zero and one, eight, two, zero, respectively. The expression of HIF- 1αand VEGF in hepatic fibrosis rats of the middle dose of Polygonum perfoliatum L from （ - ） to （3 ＋ ） were one ,seven, two, zero and one, six, three, zero, respectively. Compared with the model group, the high and middle dose of Polygonum perfoliatum L can redilce the expression of HIF-1αand VEGF in hepatic fibrosis rats （ P 〈 0. 01 ）. Conclusions Polygonum perfoliatum L has a good curative effect of anti hepatic fibrosis. Its therapeutic mechanism mainly maybe due to adjust the metabolism of extracellular matrix by decreasing HIF-1αand VEGF expression.