产妇血及胎盘组织转化生长因子β1异常表达与预测胎儿生长受限的研究

Study on abnormal expression of transforming growth factor-β1 in maternal blood and placenta of patients with fetal growth restriction

目的探讨产妇血及胎盘组织转化生长因子β1（TGF—β1）表达与胎儿生长受限（FGR）的关系。方法采用酶联免疫吸附法（ELISA）和免疫组织化学方法检测33例FGR孕妇和120例正常孕妇血及胎盘组织TGF-β1水平，比较两组孕妇血及胎盘组织TGF-β1水平变化及阳性和强阳性表达。结果TGF—β1阳性颗粒主要表达于绒毛中的合体滋养层细胞中的胞浆，细胞滋养层细胞和绒毛间质细胞未见TGF-β1表达，而蜕膜细胞呈弱阳性表达。FGR组孕妇血清中TGF-β1水平（76．5±33．4）高于对照组（47．6±24．2，t’=4．65，P〈0．05）。TGF-β1在两组孕妇绒毛组织合体滋养层细胞表达阳性率为100％，但合体滋养层细胞中TGF-β1强阳性率（81．82％）高于正常妊娠组（5．83％，P〈0．01），两组孕妇蜕膜细胞中TGF-β1阳性率均为100％（P〉0．05）。结论胎儿生长受限与TGF-β1水平高低有关，动态监产妇血TGF-β1水平有利预测胎儿生长受限，对临床防治具有指导意义。

Objective To find out the relationship between fetal growth restriction （FGR） and transforming growth factor -β1（TGF-β1）. Methods The levels of TGF-β1 in matemat serum and plaeental tissue of FGR were detected with using ELISA and immunohistochemistry technique, and it was compared with those of normal term pregnancy. Results The levels of TGF-β1 in maternal serum of FGR group were significantly higher than those of normal term pregnancy （ 76. 5 ± 33.4 VS 47. 6 =t 24. 2, t＇ = 4. 65, P 〈 0. 05）. As for the intensity of the immunohistoehemistry signal, TGF-β1 in syncytiotmphoblastic cells of FGR was markedly higher than that of control group （81.82% vs 5.83%, P 〈 0. 01 ）. Conclusions The levels of TGF--β1 are elosely related with FGR.