精子顶体反应检测在宫腔内人工授精中的临床应用价值

Sperm acrosome reaction detection in intrauterine insemination

目的:探讨精子顶体反应(AR)与宫腔内人工授精(IUI)临床妊娠率的关系。方法:对接受IUI治疗的128例患者检测自发AR率、Ca2+载体A23187诱发AR率,收集临床资料,分析精子AR率与IUI临床妊娠率的关系。结果:妊娠组的自发AR率为7.7%,诱发AR率为51.9%,非妊娠组的自发AR率为7.0%,诱发AR率为43.5%,2组自发AR率差异无统计学意义(P>0.05),诱发AR率差异有统计学意义(P<0.05);根据诱发AR率(≤20.0%,20.1%～49.9%和≥50.0%)将所有患者分为3组,其临床妊娠率分别为4.8%,12.5%和18.6%,差异有统计学意义(P<0.05)。结论:自发AR率与IUI临床妊娠率无关,诱发AR率与IUI临床妊娠率有关。

Objective To investigate the relationship between sperm acrosome reaction（AR） and the clinical pregnancy rate of intrauterine insemination（IUI）.Methods We detected the sperm spontaneous AR rate and Ca2＋ ionophore A23187-induced AR rate in 128 patients who accepted IUI treatment,collected their clinical data and analysed the relationship between sperm AR rate and clinical pregnancy rate of IUI.Results There was no statistical difference between the spontaneous AR rates in the pregnant group and the non-pregnant group（7.7% vs.7.0%,P0.05）,but there was statistical difference between the induced AR rates（51.9 % vs.43.5%,P0.05）.There was statistical difference in the clinical pregnancy rate among the 3 IUI groups divided by induced AR rate（≤20.0%,20.1%-49.9%,and ≥50.0%;4.8% vs.12.5% vs.18.6%,P0.05）.Conclusion The spontaneous AR rate has nothing to do with the clinical pregnancy rate of IUI,but the induced AR rate is associated with the clinical pregnancy rate of IUI.