口腔扁平苔藓差异表达MicroRNAs的筛选

Identification of differentially expressed MicroRNAs in oral lichen planus

目的:芯片筛选口腔扁平苔藓(oral lichen planus,OLP)损害黏膜中差异表达的miRNAs。方法:收集白纹型OLP损害黏膜和正常口腔黏膜各2例,抽提总RNA预扩增后运用Taqman低密度芯片(v2.0)测定人类667种miRNAs的表达谱,随后应用实时定量PCR在68例样本中进一步验证miR-27b的表达水平。采用miRNAs生物信息库预测miR-27b所对应的靶基因,对芯片数据进行统计学分析。结果:筛选获得30个OLP相关的miRNAs。相对于正常口腔黏膜,OLP损害黏膜中有18个miRNAs表达下调,12个上调。扩大样本量至68例验证miR-27b表达水平,结果与芯片实验一致。生物信息学分析得出miR-27b的预测靶基因中有10个与OLP发病有关。结论:筛选得到的OLP损害黏膜中差异表达的miRNAs可能与OLP的发生相关。

Objective： To identify the differently expressed MicroRNAs（miRNAs） profile in oral lichen planus（OLP）.Methods： Total RNA was extracted from the mucosa of 2 patients with reticular OLP and 2 normal mucosa samples respectively.For the miRNAs expression analysis,after PCR,TaqMan Low Density Array v2.0 was used to detect the differential expression profile of 667 human miRNAs.Real-time quantitative PCR was applied to further verify the relative expression level of miR-27b in 68 samples.Then 3 online database for miRNAs（MicroRNA,Targetscan,Pictar） were adopted to predict the possible target genes for miR-27b.Results： 30 OLP-related miRNAs were obtained.In OLP,18 down-regulated and 12 up-regulated miRNAs were found.The expressive tendency of miR-27b in 68 samples verified by Real-time quantitative PCR was in accordance with microarray.Moreover,10 OLP-related target genes were selected for miR-27b.Conclusion： The differential expression miRNAs in OLP mucosa was obtained,which may be related to the pathogenesis and development of OLP.