TGF-β1原核表达载体的构建

CONSTRUCTION OF PROKARYOTIC EXPRESSION VECTOR OF TGF-β 1

目的:克隆人TGF-β1基因,为TGF-β1的研究提供平台。方法:应用RT-PCR技术扩增人TGF-β1基因序列,将TGF-β1基因插入载体PET-28a,构建pET-28a-TGF-β1重组质粒。结果:经限制性内切酶鉴定及DNA序列测定,重组质粒PET-28a-TGF-β1序列及阅读框架正确。结论:获得了TGF-β1编码序列和表达载体,能满足进一步实验的需要。

Objective：To clone human TGF - β1 gene which lays a good foundation for TGF - β1 experiment. Methods：Using RT - PCR technology to amplified human TGF - β1 gene sequence. Then the target gene was cloned into a prokaryotic expression vector pET -28a by using the recombinant DNA technique, construction recombinant plasmid of PET- 28a -TGF - β1. Results： Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA, the results were in correspondence with the initial design. Conclusion：The encoding sequence and expression vector of TGF - β1 was obtained and can meet. Able to meet the need for further experiments.