摘要
研究了甘氨酸与乙酰丙酮－甲醛试剂显色反应的最佳条件、反应机理及共存物质的影响等。开发出食品中添加剂甘氨酸的比色分析方法。在ｐＨ６．５乙酸盐介质中，显色产物的λｍａｘ为４１５ｎｍ，甘氨酸含量在０～２００μｇ／１０ｍＬ范围内符合比尔关系，摩尔吸光系数ε为２．３×１０＾３Ｌ／ｍｉｎ·ｃｍ。
In this paper,the optimum condition of colour reaction of glycine with acetylacetone-formaldehyde reagent in pH 6.5 HOAc-NaOAc medium has been studied as well as the reaction mechanism and the influence of coexisting substances.Beer's law is obeyed in the rangen of 0 ~ 200μg/10 mL of glycine at 415 nm.The molar absorptivity was 2.3×103L/mol·cm.
出处
《化学分析计量》
CAS
1999年第1期18-19,共2页
Chemical Analysis And Meterage
基金
河北省科委基金
关键词
甘氨酸
食品添加剂
比色法
测定
glycine,acetylacetone-formaldehyde,food,colorimetry
