摘要
目的:建立测定热淋清片中没食子酸含量的方法。方法:采用反相高效液相色谱法。色谱柱为C18柱,流动相为甲醇0.1-601.14%4～磷1酸.6(1424∶9μ8g)范,检围内测与波峰长面为积27积3分nm值,呈流良速好为的1线.0性mL关·系m(inr-=1,0进.99样9量9,n为=150);μ平L,均柱加温样为回3收0率℃为。9结8.果87%:没(R食SD子=酸0.进77样%量,n在=6)。结论:该法简便、准确,可用于热淋清片的质量控制。
OBJECTIVE：To establish a method for the content determination of gallic acid in relinqing tablets. METHODS： RP-HPLC method was adopted. The determination was performed on C18 column with mobile phase consisted of methanol-0.1% phosphoric acid （2 ∶ 98） at the detection wavelength of 273 nm. The flow rate was 1.0 mL·min-1 and injection volume was 10 μL. The column temperature was 30 ℃. RESULTS： The linear range of gallic acid was 0.161 44～1.614 4 μg（r=0.999 9, n=5） with an average recovery of 98.87%（RSD=0.77%, n=6）. CONCLUSION： The method is simple and accurate for the analysis of gallic acid in relinqing tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第32期3052-3054,共3页
China Pharmacy
