β-珠蛋白生成障碍性贫血患者外周血单个核细胞B细胞淋巴瘤/白血病11A基因mRNA表达特点

Expression Characteristics of B-Cell Lymphoma/Leukemia 11A mRNA in Peripheral Blood Mononuclear Cells of Patients with β-Thalassemia

目的探讨β-珠蛋白生成障碍性贫血（β-地贫）患者外周血单个核细胞（PBMCs）中B细胞淋巴瘤/白血病11A（BCL11A）基因的表达特点。方法入选35例β-地贫患者为病例组;40例健康体检者为健康对照组,并进行红细胞计数。提取新鲜外周血PBMCs中总RNA,反转录为cDNA,采用荧光染料（SYBR GreenⅠ）实时荧光定量PCR（RT-PCR）和相对定量分析方法,以GAPDH基因为内参,检测2组PBMCs中BCL11A基因的表达情况。采用相对定量2-△△Ct法进行mRNA相对表达量比较,不同样本之间的相对表达量（%）=2-△Ct×100%。BCL11A基因mRNA相对表达量和红细胞计数、年龄和性别的相关分析中双变量符合正态分布的计量资料采用Pearson相关分析,其他变量采用Spearman相关分析。结果 RT-PCR检测BCL11A基因mRNA在2组中的表达,健康对照组BCL11A基因表达水平是病例组的2.46倍,2组比较差异有统计学意义。病例组中BCL11A基因mRNA的相对表达量为0.31%~11.60%;健康对照组中BCL11A基因mRNA的相对表达量为1.40%~26.70%,存在明显的个体差异。BCL11A基因mRNA的相对表达量与RBC计数（r=-0.21,P=0.930）、性别（r=-0.20,P=0.842）、年龄（r=-1.15,P=0.256）均无相关性。结论β-地贫患者BCL11A基因表达下降,BCL11A基因低表达可能在γ珠蛋白基因持续表达过程中发挥了一定作用。

Objective To explore the characteristics of B-cell lymphoma/leukemia11A（BCL11A） expressions in peripheral blood mononuc-lear cells（PBMCs） in patients with β-thalassemia,to find out features of BCL11A expression.Methods Thirty-five patients with β-thalassemia were assigned into case group.Forty healthy individuals were assigned into healthy control group.Red blood cell（RBC） counts were also tested.Total RNA samples from fresh PBMCs in the peripheral blood were reverse transcribed into cDNA.Glyceraldehyde-3-phosphate dehydrogenase（GAPDH） was used to internal reference gene.SYBR Green Ⅰ real-time fluorescence quantitative PCR（RT-PCR） and relative quantitative analysis method were performed to detect the mRNA expressions of BCL11A in PBMCs of 2 groups.Relative expressions of mRNA in 2 groups were compared by 2-△△Ct method of relative quantification.Relative expression between different samples（%） were calculated by 2-△Ct×100%.Bivariate correlation analysis between BCL11A gene mRNA relative expression with RBC count,age and sex were used.Normal distribution of measurement data were calculated statistically by Pearson correlation analysis and the other data were statisticed by Spearman correlation analysis.Results The values of BCL11A expression in healthy control group was 2.46 times higher than case group.In case group,the relative expression of BCL11A mRNA was 0.31%-11.60%.In the control group,the relative expression of BCL11A mRNA was 1.40%-26.70%.Individual differences in relative expression in BCL11A mRNA were obvious.Relative expression of BCL11A gene mRNA did not associated with RBC count（r=-0.21,P=0.930）,sex（r=-0.20,P=0.842）or age（r=-1.15,P=0.256）.Conclusions The levels of BCL11A expression may decrease in patients with β-thalassemia.Low expression of BCL11A may be contributable to persistent expression of γ-globin gene.