寇氏隐甲藻不同破壁方法的研究

Study on Different Wall-broken Methods for Crypthecodinium Cohnii

寇氏隐甲藻是海洋微藻的一种,在寇氏隐甲藻的细胞油脂中,二十二碳六烯酸的含量高达30%～50%。但长期以来,因为微藻的细胞壁很难破碎,导致油脂的提取率不高。运用超声波法、生物酶法,反复冻溶法等方法,对寇氏隐甲藻进行了破壁研究。结果表明,这些方法都对寇氏隐甲藻都具有一定的破壁作用,破壁后油脂的提取率也有了不同程度的提高,把酶法破壁和超声波法破壁结合起来,在酶解5 h后,再超声波1 h,破壁率可高达91.7%,油脂提取率提高了21.7%,破壁效果更为理想。

Crypthecodinium cohnii is a marine microalgae , and the DHA contents are as high as 30% to 50% in the Crypthecodinium cohnii cell oils. But for a long range, oil extraction rate was unsatisfactory since cell walls were hard to break. The breaking of cell wall of Crypthecodinium cohnii by supersonic treatment, enzymatic procession and temperature - difference was evaluated separately in the article. The results showed that all methods were effective to the wall - broken efficiency and oil extraction rate. The better results could be obtained if we combined the enzymatic procession（for 5 h, first） and the supersonic treatment （for 1 h, then）, as the rate of wall broken could be up to 91.7% and the oil extraction rate increased 21.7%.