Zebularine对K562细胞的增殖及凋亡的影响

Effect of Zebularine on the Proliferation and Apoptosis of K562 Cells

【目的】探讨药物zebularine对白血病细胞株K562增殖及凋亡的影响。【方法】选择白血病细胞株K562，试验分为对照组（未加入zebularine组）、实验组（zebularine终浓度分别为250,aM，500／tM）组3个组。MTT试剂盒检测12h、24h、36h、48h和72h后K562细胞株OD570；用AnnexinV／FiTC试剂盒与细胞周期检测试剂盒检测k562细胞在药物干预48h后的细胞凋亡情况。【结果】k562细胞株在zebularine干预48h后，随着zebularine处理浓度的增加，细胞活性明显降低（P〈0．01）；K562细胞株在zebularine干预48h后，药物浓度250弘M、500pM组与对照组相比，早期凋亡细胞比例与细胞凋亡峰（APO峰）值明显增大，差异有显著性（P〈0．01），500pM组与250uM组相比，早期凋亡细胞比例与细胞凋亡峰（APO峰）值明显增大，差异有显著性（P〈0．01）。【结论】Zebularine可抑制K562细胞株的增殖，促进其凋亡，并有一定的浓度依赖性。

[Obiective] To understand the effect of zebularine on the proliferation and apoptosis of leukemia cells K562 and explore its tumor suppressor mechanism and value in the treatment of leukemia. [Methods] The K562 leukemia cells were selected and divided into the control group（without zebulaine） and the experimental groups（zebularine with the final concentration of 250μM and 500μM, respectively）. MTT assay kit was used to detect the OD570 of K562 cells after 12h, 36h, 24h, 48h and 72h. Annexin V/FITC kit and cell cycle kit were used to detect the apoptosis of K562 cells at 48h after drug intervention. [Results] At 48h after drug intervention, the activity of k562 markedly reduced with the increasing of concentration of zebularine（ P 〈0.01）. Compared with the control group, the proportion of early apoptotic cells and apoptotic peak（APO） value in the experimental groups after 48h of drug intervention increased obviously, but there was significant difference（ P 〈0.01）. Compared with 250μM group, the proportion of early apoptotic cells and APO peak value of 500μM group increased obviously, and there was significant difference between two groups （ P 〈0.01）. [Conclusion]Zebularine can inhibit the proliferation of K562 cells and promote the apoptosis in a dosedependent manner.