南方型紫花苜蓿基因型在组织培养条件下对NaCl的耐性

NaCl tolerance of different genotypes of southern type of Medicago sativa under tissue culture

以4个南方型紫花苜蓿基因型为材料,通过测定愈伤组织生长状况、组织培养条件下的发芽率、苗高和幼根长指标,研究南方型紫花苜蓿对NaCl的耐受性。结果表明:NaCl对4个南方型紫花苜蓿的生长指标的影响均存在较大差异,其中愈伤组织生长和发芽率受NaCl影响小,幼根生长对NaCl的耐受性较大,120 mmol.L-1NaCl严重影响了南方型紫花苜蓿幼苗生长。当培养基中加入NaCl时,‘Millennium’和‘Vito-ria’的耐受性较强,而‘Powerplant’的耐受性较差,在进行南方型紫花苜蓿遗传转化的筛选时,应注意不同基因型和受体类型对NaCl的耐受性。

Four genotypes of southern type Medicago sativa were used as experimental materials to investigate their NaCl tolerance by determing callus growth,germination rate,seedling height and radicle length under tissue culture condition.The results showed that NaCl tolerance was different among different genotypes of southern type Medicago sativa.The callus growth and germination rate were rarely affected under NaCl stress while radicle length was greatly affected under NaCl stress.When NaCl concentration was 120 mmol·L^-1,the seedling growth of southern type of Medicago sativa was obviously affected.＇Millennium＇ and ＇Vitoria＇ showed higher NaCl tolerance,but the NaCl tolerance of ＇Powerplant＇ was the weakest.Difference in NaCl tolerance among different genotypes should be noticed when transferred southern type Medicago sativa plants were selected.