GC法检测三类食品中富马酸二甲酯的含量

Determination of dimethyl fumarate in three types of food by gas chromatography

选取饮料、饼干、腌菜三类保质期较短的食品为研究对象,试验了不同提取溶剂、提取方法、色谱条件的影响,建立了气相色谱法测定食品中富马酸二甲酯残留量的检测方法。试样经三氯甲烷提取后,超声15min,DB-FFAP柱分离,氢火焰离子化检测器(FID)检测。富马酸二甲酯浓度在10～600μg.mL-1范围内,标准曲线的回归方程为y=24825x,相关系数r=0.9999,检出限0.072 mg.kg-1,分别对饮料、饼干、腌菜三类样品进行高低两水平添标回收试验,平均回收率在87.8%～105.2%,相对标准偏差2.4%～4.5%(n=6),本方法操作简单、快速、准确,灵敏度高,重现性好,适用于食品中富马酸二甲酯的检测。

Gas chromatography（GC） method was developed for the determination of dimethyl fumarate（DMF）in food including drinks,biscuits and pickles sample.The effects of different extraction solvent,different extraction means and different chromatographic conditions were investigated.Samples could be extracted from food sample by chloroform and ultrasonic,and then were isolated by DB-FFAP column,detected by FID.The standard curve of DMF showed good linearity in the concentration range of 10-600 μg·mL-1（r=0.9999）.The detection limit of DMF was 0.072 mg·kg-1.The average recoveries of imazalil from three matrices at high and low levels ranged from 87.8% to 105.2% with DMF,and the repeat nature with relative standard deviations（RSD） was 2.4%-4.5%.In conclusions,this method is sensitive,simple accurate for the control of DMF in food.