摘要
目的建立反相高效液相色谱法(reversed phase-high performance liquid chromatography,RP-HPLC)测定盐酸美普他酚(2-氨基-6-甲基)-苯甲酸酯的含量。方法采用C18(4.6 mm×150 mm,5μm)色谱柱,0.02 mol/L醋酸铵水溶液(氨水调节pH至7.4):甲醇(25∶75,V/V)为流动相,流速1.0 mL/min,检测波长230 nm,柱温30℃。结果盐酸美普他酚(2-氨基-6-甲基)-苯甲酸酯的最低定量限为20 ng,线性范围20-500μg/mL(r=0.999 4)。结论本方法简便、灵敏、准确,可用于盐酸美普他酚(2-氨基-6-甲基)-苯甲酸酯的质量控制。
Objective To establish a reversed phase-high performance liquid chromatography(RP-HPLC) method to determine the content of meptazinol(2-amino-6-methyl)-benzoate hydrochloride.Methods A C18(4.6 mm×150 mm,5 μm) column was used with the mobile phase of 0.02 mol/L ammonium acetate(pH adjusted to 7.4 with aqueous ammonia): methanol(25 ∶ 75,V/V),the flow rate of 1.0 mL/min,the UV-detector at the wavelength of 230 nm,and the column temperature of 30 ℃.Results The limit of detection for meptazinol(2-amino-6-methyl)-benzoate hydrochloride was 20 ng.Good linearity was demonstrated at the range of 20-500 μg/mL(r=0.999 4).Conclusions The established method was proved to be simple,sensitive and accurate.It can be used for the quality control of meptazinol(2-amino-6-methyl)-benzoate hydrochloride.
出处
《复旦学报(医学版)》
CAS
CSCD
北大核心
2011年第4期337-339,共3页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金项目(30271539
30801435)
高等学校博士学科点专项科研基金项目(新教师基金
200802461095)