期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

外源性TGF-β1诱导人乳腺癌MCF-7细胞的乙醛脱氢酶1 mRNA及蛋白表达 被引量：3

Exogenous TGF-β1 upregulates the expression of ALDH1 in human breast cancer MCF-7 cells
下载PDF
导出
摘要 目的本研究旨在探讨外源性TGF-β1诱导人乳腺癌MCF-7细胞对乙醛脱氢酶1(aldehyde dehydrogenase1,ALDH1)mRNA及蛋白表达的影响。方法用不同浓度(1、5ng/mL)的TGF-β1诱导体外培养的MCF-7细胞48h后,RT-PCR检测ALDH1mRNA的表达变化,Western blot检测ALDH1蛋白的表达变化。结果 TGF-β1(1、5ng/mL)诱导的MCF-7细胞的ALDH1mRNA及蛋白表达均高于未诱导的MCF-7亲本细胞,差异有统计学意义(P均<0.001);高浓度(5ng/mL)TGF-β1诱导的MCF-7细胞的ALDH1mRNA及蛋白表达均高于低浓度(1ng/mL)TGF-β1诱导的MCF-7细胞,差异有统计学意义(P均<0.001)。结论外源性TGF-β1可诱导人乳腺癌MCF-7细胞上调ALDH1mRNA及蛋白的表达,可能诱导ALDH1+乳腺癌肿瘤干细胞(cancer stemcell,CSC)的转化,并有一定的浓度依赖性。 Objective To investigate the effects of exogenous TGF-β1 on the regulation of aldehyde dehydrogenase 1（ALDH1） expression in human breast cancer MCF-7 cells.Methods MCF-7 cells were induced with TGF-β1 at different concentrations（1,5 ng/mL） for 48 hours.ALDH1 expression in these cells was then analyzed at mRNA and protein levels by RT-PCR and Western blot assay,respectively.Results ALDH1 mRNA and protein expressions were up-regulated in TGF-β induced（1,5 ng/mL） MCF-7 cells compared with normal MCF-7 cells,respectively（All P0.001）.They were also up-regulated in MCF-7 cells induced with TGF-β1 at higher concentration（5 ng/mL） compared with MCF-7 cells induced with TGF-β1 at lower concentration（1 ng/mL）,respectively（All P0.001）.Conclusions Exogenous TGF-β1 up-regulats expressions of ALDH1 mRNA and protein in human breast cancer MCF-7 cells in the manner of concentration-dependence,and TGF-β1 may mediates the convention of cancer stem cells（CSC） to breast cancer cells.
作者 韩明利 吴诚义 莫志强 彭琼乐 王艺梦 屈洪波
机构地区 重庆医科大学附属第一医院内分泌外科 重庆医科大学基础医学院细胞生物学及遗传学教研室 重庆医科大学基础医学院分子医学与肿瘤研究中心 重庆医科大学附属第一医院急诊科
出处 《复旦学报（医学版）》 CAS CSCD 北大核心 2011年第4期340-342,共3页 Fudan University Journal of Medical Sciences
关键词 TGF-Β1 乙醛脱氢酶1 乳腺癌 上皮-间质转化 肿瘤干细胞 TGF-β1 aldehyde dehydrogenase 1 breast cancer epithelial-mesenchymal transition cancer stem cell
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1Papageorgis P, Lambert AW, Ozturk S, et al. Smad signaling is required to maintain epigenetic silencing during breast cancer progression [J].Cancer Res, 2010,70(3) :968 - 978.
  • 2Gregory PA, Bert AG, Paterson EL, et al. The miR- 200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 [J]. Nat Cell Bio1,2008,10(5) : 593- 601.
  • 3Zheng Q, Safina A, Bakin AV. Role of high-molecular weight tropomyosins in TGF-beta-mediated control of cell motility[J]. Int J Cancer,2008,122(1) :78 - 90.
  • 4Mani SA, Guo W, Liao MJ, et al. The epithelial- mesenchymal transition generates cells with properties of stem cells[J]. Cell ,2008,133(4):704- 715.
  • 5Morel AP, Lievre M, Thomas C, et al. Generation of breast cancer stem cells through epithelial-mesenchymal transition[J]. PLoS One, 2008,3 (8) : e2 888.
  • 6Santisteban M, Reiman JM, Asiedu MK, et al. Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells[J]. Cancer Res,2009,69(7) :2 887-2 895.
  • 7AI-Hajj M, Wicha MS, Benito-Hernandez A, et al. Prospective identification of tumorigenic breast cancer cells[J]. Proc Natl Acad Sci USA, 2003, 100 (7) : 3983-3988.
  • 8Ginestier C, Hur MH, Charafe-Jauffret E, et al. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome[J]. Cell Stem Cell, 2007,1 (5) : 555 - 567.
  • 9Wicha MS. Cancer stem cells and metastasis: lethal seeds [J]. Clin Cancer Res ,2006,12(19) :5 606 - 5 607.
  • 10Bomken S, Fiser K, Heidenreich O, et al. Understanding the cancer stem cell[J]. Br J Cacer ,2010,103(4) :439 - 445.

同被引文献28

  • 1Perou CM, Sorlie T, Eisen MB, et al. Molecular portraits of human breast tumours. Nature, 2000, 406(6797):747-752.
  • 2Mahler-Araujo B, Savage K, Parry S, et al. Reduction of E-eadherin expression is associated with non-lobular breast carcinomas of basal-like and triple negative phenotype. J Clin Pathol, 2008, 61(5): 615-620.
  • 3Lascombe I, Clairotte A, Fauconnet S, et al. N-cadherin as a novel prognostic marker of progression in superficial urothelial tumors. Clin Cancer Res, 2006, 12(9):2780-2787.
  • 4AI-Hajj M, Wicha MS, Benito-Hernandez A, et al. Prospective identification of tumorigenic breast cancer cells. Proc Nad Acad Sci U S A, 2003, 100(7):3983-3988.
  • 5Ginestier C, Hur MH, Charafe-Jauffret E, et al. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell, 2007, 1(5):555-567.
  • 6Hay ED. The extracellular matrix in development and regeneration. An interview with Elizabeth D. Hay. Int J Dev Biol, 2004, 48(8-9): 687-694.
  • 7Wheelock MJ, Shintani Y, Maeda M, et al. Cadherin switching. J Cell Sci, 2008, 121(Pt 6):727-735.
  • 8Morel AP, Li6vre M, Thomas C, et al. Generation of breast cancer stem cells through epithelial-mesenchymal transition. PLoS One, 2008 3(8):e2888.
  • 9Mani SA, Guo W, Liao Mr, et al. The epithelial-mesenchymal transition generates ceils with properties of stem cells. Cell, 2008, 133(4):704-715.
  • 10周凌,虞佩,王建丰,宋科瑛,江爱芳,徐红,李克.乙醛脱氢酶1蛋白在乳腺癌中的表达及临床意义[J].肿瘤,2009,29(7):663-667. 被引量：21

引证文献3

二级引证文献7

复旦学报（医学版）
2011年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部