采用UPLC-ESI-MS快速测定博落回各器官中主要生物碱的含量

Rapid determination of main alkaloids from organs of Macleagua cordata(Willd) R.Br.by UPLC-ESI-MS

采用超高效液相色谱-电喷雾质谱联用（UPLC-ESI-MS）技术,对中药博落回各器官中的主要生物碱成分进行系统分析。样品经超声萃取后,通过UPLC-ESI-MS快速测定了8种生物碱在根、茎、叶和果荚中的含量,获得相应化合物的保留时间、相对分子质量和离子碎片等信息,并从博落回果荚中分离、鉴定隐品碱。在此基础上,建立一种快速、灵敏、准确测定博落回中多种生物碱成分的新方法。研究结果表明：这8种生物碱含量在一定范围内呈线性关系,相关系数为0.992 5~0.998 3,平均加标回收率为96.6%~104.0%,相对标准偏差均小于4.3%,检出限为0.123~1.270μg/L。

An ultra-performance liquid chromatography-electrospray ionization mass spectrometry（UPLC-ESI-MS） analysis method was developed for systematic determination of the main alkaloids in the organs of Macleagua cordata（Willd） R.Br..After a simple ultrasound-assisted extraction step,the proposed method was applied to quantify eight alkaloids in the roots,stems,leaves and fruits of Macleagua cordata.The retention time,relative molecular mass and fragmentation behavior were obtained.A new compound of cryptopine was separated and identified from the fruits of the plant with literature review.The results show that the correlation coefficients is 0.992 5？0.998 3 in the linear range.The average recovery of the investigated alkaloids is 96.6%？104.0%,and the relative standard deviation is less than 4.3%,with the lower limit of detection values of 0.123？1.270 μg/L.The present method has the advantages of simple operation and rapid determination,and it is a powerful tool for quantification of alkaloids in M.cordata.