抗-HCVELISAS/CO比值与确证试验阳性的相关性探讨

Study on the correlation of the anti-HCV ELISA S/CO ratio and the positive results of confirmatory test

目的探讨抗-HCVELISAS/CO比值与抗-HCV免疫印迹法(RIBA)确证试验阳性的相关性。方法分别采用进口和国产试剂同时筛查34504例无偿献血者血液,结果有反应性标本用RIBA确证试剂进行确证,分析两种ELISA试剂有反应性S/CO阈值及其对确证试验阳性结果的预测率。结果 34504例标本中抗-HCV有反应性92例。国产试剂筛查出有反应性57例,经RIBA确证阳性30例,其中S/CO≥10.0的RIBA确证阳性率96.70%(29/30)明显高于S/CO<10.0的3.70%(1/27),两者差异有统计学意义(χ2=45.60,P<0.0001);进口试剂有反应性71例,经RIBA确证阳性31例,其中S/CO≥4.0的RIBA确证阳性率96.80%(30/31)明显高于S/CO<4.0的2.50%(1/40),两者差异有统计学意义(χ2=60.36,P<0.0001)。两种试剂同时有反应性标本36例,经RIBA确证阳性30例。结论抗-HCVELISAS/CO比值与确证试验阳性有一定的相关性,通过S/CO比值可以预测抗-HCV阳性。

Objective To investigate the correlation of the anti-HCV ELISA S/CO ratio and the positive results of confirmatory test.Methods 34 504 samples of volunteer blood donors were screened with imported and domestically diagnostic kits.Positive results were confirmed with RIBA test.The ELISA S/CO ration and its prediction for the positive results in the confirmatory test were analyzed.Results In 34 504 cases,92 were anti-HCV positive,of which 57 positive cases were detected by domestic diagnosis kit and 30 of such cases were S/CO≥10.0,and they were positive in confirmatory test.There were 71 positive cases detected by imported diagnostic kit,31 of such cases were S/CO≥4.0,and they were confirmed positive.36 cases were detected positive by both diagnostic kits,and 30 of them were confirmed by RIBA.Conclusion The anit-HCV ELISA S/CO ratio is correlated with confirmation positive,so ELISA S/CO ratio can be used to predict anti-HCV real positive.