Effects of GLUT4 expression on insulin resistance in patients with advanced liver cirrhosis 被引量：2

Effects of GLUT4 expression on insulin resistance in patients with advanced liver cirrhosis

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摘要 Decreased glucose tolerance and diabetes are frequently observed in advanced liver cirrhosis patients and may be related to insulin resistance.Glucose transporter-4 (GLUT4),one of the most important glucose transporters,plays a key role in the development of type 2 diabetes.In order to study the mechanism of insulin resistance in liver cirrhosis patients,we measured the insulin sensitivity index and determined the GLUT4 protein and mRNA contents of skeletal muscle by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR),respectively,in normal people and liver cirrhosis patients.The results showed that the levels of glucose,insulin,and C-peptide in two liver cirrhosis groups were higher and the insulin sensitivity index lower than those of the normal control group.The sensitivity of insulin may decrease with the decline of liver function.However,the contents of GLUT4 protein and mRNA in patients with advanced liver cirrhosis were similar to those of normal controls.In conclusion,insulin resistance is observed in patients with advanced liver cirrhosis but may not be correlated with the skeletal contents of GLUT4 protein and mRNA. Decreased glucose tolerance and diabetes are frequently observed in advanced liver cirrhosis patients and may be related to insulin resistance. Glucose transporter-4 （GLUT4）, one of the most important glucose transporters, plays a key role in the development of type 2 diabetes. In order to study the mechanism of insulin resistance in liver cirrhosis patients, we measured the insulin sensitivity index and determined the GLUT4 protein and mRNA contents of skeletal muscle by Western blotting and reverse transcription-polymerase chain reaction （RT-PCR）, respectively, in normal people and liver cirrhosis patients. The results showed that the levels of glucose, insulin, and C-peptide in two liver cirrhosis groups were higher and the insulin sensitivity index lower than those of the normal control group. The sensitivity of insulin may decrease with the decline of liver function. However, the contents of GLUT4 protein and mRNA in patients with advanced liver cirrhosis were similar to those of normal controls. In conclusion, insulin resistance is observed in patients with advanced liver cirrhosis but may not be correlated with the skeletal contents of GLUT4 protein and mRNA.

作者 Wei-feng SHAN Bing-quan CHEN Shao-jun ZHU Li JIANG Yan-feng ZHOU

机构地区 Department of Anesthesiology

出处《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第8期677-682,共6页 浙江大学学报（英文版）B辑（生物医学与生物技术）

关键词葡萄糖 transporter-4 (GLUT4 ) 肝肝硬化胰岛素抵抗骨胳的肌肉 Glucose transporter-4 （GLUT4）, Liver cirrhosis, Insulin resistance, Skeletal muscle

分类号 R587 [医药卫生—内分泌] R575.2 [医药卫生—消化系统]

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1Anty, R., Ialmelli, A., Patouraux, S., Bonnafous, S., Lavallard, V.J., Senni-Buratti, M., Amor, I.B., Staccini-Myx, A., Saint-Paul, M.C., Berthier, F., et al., 2010. A new composite model including metabolic syndrome, alanine aminotransferase and cytokeratin-18 for the diagnosis of non-alcoholic steatohepatitis in morbidly obese patients. Aliment. Pharmaeol. Ther., 32(11-12):1315-1322. [doi: 10.1111/j. 1365-2036.2010.04480.x].
2Arai, H., Awane, N., Mizuno, A., Fukaya, M., Sakuma, M., Harada, N., Kawaura, A., Yamamoto, H., Okumura, H., Taketani, Y., et al., 2010. Increasing early insulin secretion compensate adequately for hepatic insulin resistance in CCl4-induced cirrhosis rats. J. Med. Invest., 57(1-2):54-61. [doi: 10.2152/jmi.57.54].
3Asano, T., Fujishiro, M., Kushiyama, A., Nakatsu, Y., Yoneda, M., Kamata, H., Sakoda, H., 2007. Role of phosphatidy- linositol 3-kinase activation on insulin action and its al- teration in diabetic conditions. Biol. Pharm. Bull., 30(9): 1610-1616. [doi:10.1248/bpb.30.1610].
4Aytug, S., Reich, D., Sapiro, L.E., Bemstein, D., Begum, N., 2003. Impaired IRS-1/PI3-kinase signaling in patients with HCV: a mechanism for increased prevalence of type 2 diabetes. Hepatology, 38(6):1384-1392. [doi:10.1053/ jhep.2003.09012].
5Braganga, A.C., Alvares-da-Silva, M.R., 2010. Prevalence of diabetes mellitus and impaired glucose tolerance in pa- tients with decompensated cirrhosis being evaluated for liver transplantation: the utility of oral glucose tolerance test. Arq. Gastroenterol., 47(1):22-27. [doi:10.1590/ S0004-28032010000100005].
6Chang, L., Chiang, S.H., Saltiel, A.R., 2004. Insulin signaling and the regulation of glucose transport. Mol. Med., 10(7-12):65-71. [doi:10.2119/2005-00029.Saltiel].
7Chen, X., AI-Hasani, H., Olausson, T., Wenthzel, A.M., Smith, U., Cushman, S.W., 2003. Activity, phosphorylation state and subcellular distribution of GLUT4-targeted Akt2 in rat adipose cells. J. Cell. Sci., 116(17):3511-3518. [doi: 10.1242/jcs.00675].
8Holland-Fischer, P., Andersen, P.H., Lurid, S., Pedersen, S.B., Vinter-Jensen, L., Nielsen, M.F., Kaal, A., Dall, R., Schmitz, O., Vilstrup, H., 2007. Muscle GLUT4 in cir- rhosis. J. Hepatol., 47(2):212-219. [doi:10.1016/j.jhep. 2007.02.012].
9Huang, S., Czech, M.P., 2007. The GLUT4 glucose transporter. Cell Metab., 5(4):237-252. [doi:10.1016/j.cmet.2007.03. 006].
10Hung, C.H., Wang, J.H., Hu, T.H., Chen, C.H., Chang, K.C., Yen, Y.H., Kuo, Y.H., Tsai, M.C., Lu, S.N., Lee, C.M.,2010. Insulin resistance is associated with hepatocellular carcinoma in chronic hepatitis C infection. World J. Gastroenterol., 16(18):2265-2271. Idol: 10.3748/wig.v16. i18.2265].