制作C57小鼠烧伤模型时Meeh常数的取值研究

The New Meeh Constant Values in C57BL/6 Mouse Model of Thermal Injury

目的:研究制作C57BL/6小鼠烧伤模型时适于计算全身体表面积(BSA)的Meeh常数(k)值。方法:24只6～12周正常C57BL/6小鼠,雌雄各半,将其随机分为8AM组、4PM组和0AM组,每组8只。实验前3d在8AM、12N、4PM、8PM、0AM和4AM称小鼠体质量。测定每只小鼠的BSA实际值,并据此计算其k值。分别应用4个常用k值(9.1、9.5、10和11.4)计算出每只小鼠的BSA计算值。结果:24h内小鼠的体质量差别有统计学意义,4PM时最低,0AM时最高;4PM组小鼠k值大于8AM组和0AM组,差别有统计学意义(P<0.05);将小鼠常用k值与本实验所得3个时点的k值分别进行比较,除常用k值9.5与0AM组k值之间差别无统计学意义外,其余比较差别均有统计学意义(P<0.05或P<0.01)。使用小鼠常用k值计算出的BSA计算值和C57BL/6小鼠的BSA实测值之间的差值最大达到了BSA实测值的23.02%。结论:制作C57BL/6小鼠烧伤模型时可按实验时间选用不同的k值,8AM时k值宜为9.617,4PM时为9.802。

Objective：To investigate the Meeh constant（k） to be used to estimate the body surface area（BSA） in C57BL/6 mouse model of thermal injury.Methods：Twenty-four C57BL/6 mice of 6-12 weeks age were randomly divided into 8 AM,4 PM and 0 AM groups.Mice were fed with normal diet until the experimental day.On the 3rd day before the experiment,mice were weighted at 8 AM,12 N,4 PM,0 AM and 4 AM.The values of BSA were measured in each mouse,and k value was calculated.The values of BSA and k of each mouse were recorded.Results：There were significant differences in body weights of mice in 24 h,and the lowest value at 4 PM and the highest value at 0 AM.The k value was significantly higher in 4 PM than that of 8 AM and 0 AM（P 0.05）.There were statistical differences between commonly used k values and the three k values studied in this research,except for 9.5 and 0 AM groups（P 0.05 or P 0.01）.The difference between the values of BSA calculated from commonly used k value and the values of BSA measured from C57BL/6 mice varied from-8.73% to 23.02%.Conclusion：In the procedure of making the thermal injury model of C57BL/6 mice,it was reliable to use 9.617 as the k value to estimate the BSA at 8 AM,but use 9.802 at 4 PM.