HIF-1α和EMT相关蛋白在胰腺癌组织中的表达及临床意义

Clinical significance of expression of HIF-1αand EMT-related factors in pancreatic ductal adenocarcinoma

目的:探讨缺氧诱导因子-1α(HIF-1α)和上皮细胞间质转化(EMT)相关蛋白在胰腺导管腺癌(PDAC)组织中的表达及临床意义.方法:采用免疫组织化学法检测92例PDAC和10例正常胰腺组织中HIF-1α、Snail、Slug、E-cadherin、N-cadherin的表达,并分析其与患者临床病理特征和预后的关系.结果:HIF-1α、Snail、Slug、E-cadherin、N-cadherin在PDAC和正常胰腺组织中表达的阳性率分别为:69.57%,69.57%,58.70%,36.96%,73.91%;10%,0%,0%,90%,0%.HIF-1α的表达与淋巴结转移有关;Snail的表达与TNM分期有关;Slug的表达与TNM分期和淋巴结转移有关;N-cadherin的表达与TNM分期、淋巴结转移和肿瘤分化程度有关.HIF-1α表达与Snail、Slug表达呈正相关(r=0.309,P=0.005;r=0.231,P=0.027);Snail表达与E-cadherin表达呈负相关(r=-0.223,P=0.033);E-cadherin表达与N-cadherin表达呈负相关(r=-0.478,P=0.000).HIF-1α、Snail和E-cadherin是患者术后独立的预后因子.结论:HIF-1α、Snail、Slug、E-cadherin、N-cadherin在PDAC的演进及预后中发挥作用.

AIM：To investigate the expression of hypoxia inducible factor-1α（HIF-1α）and epithelial-mesenchvmal transition（EMT）-related factors in pancreatic ductal adenocarcinoma（PDAC）and to analyze their clinical significance. METHODS：Immunohistochemistry was used to examine the expression of HIF-1α,Snail,Slug, E-cadherin and N-cadherin in 92 cases of PDAC and 10 cases of normal pancreatic tissue.RESULTS：The positive expression rates of HIF-1α,Snail,Slug,E-cadherin and N-cadherin were 69.57%,69.57%,58.70%,36.96%and 73.91%in PDAC,and 10%,0%,0%,90%and 0%in normal pancreas tissue.HIF-1αexpression was significantly associated with lymph node metastasis. Snail expression was correlated with TNM stage.Slug expression was positively associated with TNM stage and lymph node metastasis. N-cadherin expression was significantly related to TNM stage,lymph node metastasis and histological grade.HIF-1αexpression in PDAC was positively correlated with Snail and Slug expression.There was a negative correlation between Snail and E-cadherin expression in PDAC.Expression of E-cadherin was negatively related to that of N-cadherin.Multivariate Cox analysis demonstrated that HIF-1α,Snail and E-cadherin were independent factors for prognosis of PDAC. CONCLUSION：HIF-1α,Snail,Slug,E-cadherin and N-cadherin play significant roles in the progression and prognosis of PDAC.