结缔组织生长因子在鼻息肉中的分布和表达

Expression of connective tissue growth factor in nasal polyps

目的探讨结缔组织生长因子(connective tissue growth factor,CTGF)与鼻息肉发生的关系。方法通过手术取鼻息肉组织(鼻息肉组)及鼻中隔偏曲患者鼻黏膜组织(对照组)。通过免疫组化法对标本进行抗人CTGF抗体染色。高倍光镜下阳性计数,计算阳性率。应用高清晰数码相机拍照,通过ImageJ图像分析系统对标本阳性细胞进行光密度测定,通过SPSS统计软件分析。结果鼻息肉组鼻黏膜下腺体细胞中CTGF阳性率高于对照组(P=0.0246<0.05),鼻息肉组鼻黏膜上皮细胞中CTGF光密度明显高于对照组(P=0.0451<0.05),鼻息肉组血管内皮细胞中CTGF光密度值高于对照组(P=0.0486<0.05)。结论鼻黏膜CTGF表达增加可能是导致鼻息肉中纤维结缔组织增多的重要原因,是鼻息肉形成的重要因素之一。

OBJECTIVE To discover the correlation between the CTGF and the development of nasal polyps.METHODS Samples were collected from the nasal polyps and normal nasal mucosa of the middle turbinate of patients during nasal operations.The slices were stained with anti-human CTG by immunohistochemistry.The percentage of positive cells were calculated under the light microscope.Analyse the optical density through the ImageJ analyse system.The data was analysed by SPSS statistics software.RESULTS The CTGF positive rate of nasal polyps group is higher than that of the contrast group in the gland cell（P=0.02460.05）.The intensity of CTGF in mucomembranous epidermis of nasal polyps group is higher than that of contrast group（P=0.04510.05）The intensity of CTGF in blood vessel endodermis of nasal polyp group is higher than that of contrast group（P=0.04860.05）.CONCLUSION The increase of CTGF expression in nasal mucosa may be the important reason of connective tissue hyperplasia in nasal polyps.It may be one of the important factor in the formation of nasal polyps.