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GIT1发夹结构对成骨细胞内局部黏附激酶的磷酸化和分布的影响 被引量：1

Effect of GIT1 hairpin on phosphorylation and distribution of focal adhesion kinase in osteoblasts
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摘要 目的探索G蛋白偶联受体激酶结合蛋白1的RNA发夹结构(GIT1-RNAh)对成骨细胞内局部黏附激酶(FAK)的磷酸化和分布的影响。方法成功构建含GFP-RNAh和GIT1-RNAh的腺病毒,在血小板衍生生长因子(PDGF)刺激的条件下,免疫荧光染色法检测成骨细胞内FAK的蛋白分布,Western blot检测FAK的磷酸化水平,Image J软件检测成骨细胞的扩增面积。结果与感染GFP-RNAh的成骨细胞相比,在PDGF刺激条件下,GIT1-RNAh明显抑制FAK磷酸化水平(P<0.05),扰乱FAK的分布,抑制成骨细胞的面积扩增(P<0.05)。结论 GIT1-RNAh通过扰乱FAK的分布及其磷酸化抑制成骨细胞的面积扩增。 Objective To investigate the effect of the RNA hairpin of G protein coupled receptor kinase interacting protein 1（GIT1-RNAh） on the phosphorylation and distribution of focal adhesion kinase（FAK） in osteoblasts.Methods The adenovirus containing GFP-RNAh or GIT1-RNAh was successfully constructed.After stimulated with platelet-drived growth factor（PDGF）,the protein distribution of FAK,phosphorylation level of FAK in osteoblasts and the amplified area of osteoblasts were detected by immunofluorescence staining,Western blot and Image J software,respectively.Results Compared with the osteoblasts infected with GFP-RNAh,GIT1-RNAh significantly inhibited the phosphorylation level of FAK（P〈0.05）,disturbed the distribution of FAK,and inhibited the amplified area of osteoblasts（P〈0.05） after PDGF stimulation.ConclusionGIT1-RNAh inhibits the amplified area of osteoblasts via disturbing the distribution and phosphory-lation of FAK.
作者 谭诗平 张宁 胡志毅 殷国勇
机构地区 沭阳县人民医院骨科 南京医科大学第一附属医院骨科
出处 《江苏医药》 CAS CSCD 北大核心 2011年第15期1745-1747,共3页 Jiangsu Medical Journal
基金 国家自然科学基金资助项目(81071481)
关键词 G蛋白偶联受体激酶结合蛋白1 RNA发夹结构 局部黏附激酶 成骨细胞 G protein coupled receptor kinase interacting protein 1 RNA hairpin Focal adhesion kinase Osteoblast
分类号 R734 [医药卫生—肿瘤]
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参考文献14

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二级参考文献19

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江苏医药
2011年 第15期

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