蓝萼香茶菜乙素对Rb细胞增殖和细胞周期的影响

Effects of glaucocalyxin B on proliferation and cell cycle of human retinoblastoma in vitro

目的探讨蓝萼香茶菜乙素对体外培养的人视网膜母细胞瘤(retinoblastoma,Rb)细胞的增殖及细胞周期的影响。方法 MTT法测定不同浓度(2.5μmol.L-1、5.0μmol.L-1、10.0μmol.L-1、20.0μmol.L-1和40.0μmol.L-1)的蓝萼香茶菜乙素作用24h及48h后对人Rb细胞株的影响;碘化丙啶染色、流式细胞术观察蓝萼香茶菜乙素对Rb细胞周期及细胞凋亡的影响。结果蓝萼香茶菜乙素不同剂量组对Rb细胞均有显著细胞毒作用。蓝萼香茶菜乙素处理24h和48h后对Rb细胞株的IC50分别为79.15μmol.L-1和10.88μmol.L-1,剂量-效应关系较为明显;对照组和不同药物浓度组(2.5μmol.L-1组、5.0μmol.L-1组、10.0μmol.L-1组、20.0μmol.L-1组、40.0μmol.L-1组)作用Rb细胞24h后,细胞凋亡率分别为:(2.01±0.03)%、(6.21±0.91)%、(10.21±2.06)%、(16.83±2.61)%、(25.17±2.11)%和(35.29±1.04)%;作用48h后,对应细胞凋亡率分别为:(2.41±0.73)%、(7.94±3.01)%、(12.08±2.43)%、(20.11±2.37)%、(35.18±3.48)%和(51.20±2.93)%。这一结果也显示:随着药物浓度增加,Rb细胞凋亡率也随之升高。结论蓝萼香茶菜乙素通过诱导Rb细胞株凋亡发挥抑制人Rb增殖的作用。

Objective To investigate the effects of glaucocalyxin B on cell proliferation and cell cycle of retinoblastoma（Rb） cell line in vitro.Methods MTT method was used to detect the effect of glaucocalyxin B with different doses（2.5 μmol·L-1,5.0 μmol·L-1,10.0 μmol·L-1,20.0 μmol·L-1 and 40.0 μmol·L-1） on growth of human Rb cells at different time points（24 hours,48 hours）.The effects of glaucocalyxin B on the cell cycle and apoptosis of Rb cells were observed by propidium iodide（PI） staining and flow cytometer,respectively.Results Glaucocalyxin B could inhibit cell proliferation in each dose group.The optical density went down with and the apoptotic rates went up with increasing of dose.The IC50 value for the inhibition of Rb cells by glaucocalyxin B for 24 hours and 48 hours were 79.15 μmol·L-1 and 10.88 μmol·L-1,the dose-effect relationship was obvious.The apoptotic rates of Rb cells after treated with glaucocalyxin B with different doses（2.5 μmol·L-1,5.0 μmol·L-1,10.0 μmol·L-1,20.0 μmol·L-1 and 40.0 μmol·L-1） for 24 hours were（6.21±0.91）%,（10.21±2.06）%,（16.83±2.61）%,（25.17±2.11）% and（35.29±1.04）%,respectively,and（2.01±0.03）% in control group,which for 48 hours were（7.94±3.01）%,（12.08±2.43）%,（20.11±2.37）%,（35.18±3.48）% and（51.20±2.93）%,respectively,and（2.41±0.73）% in control group.The apoptotic rate of Rb cells was obviously increased after treated with glaucocalyxin B with a certain dose dependent manner.Conclusion Glaucocalyxin B can inhibit the proliferation of Rb by inducing the apoptosis of Rb cell strains.