细胞角蛋白17在口腔鳞癌中的表达

Expression of cytokeratin 17 in oral squamous cell carcinoma

目的研究细胞角蛋白17(CK17)在口腔鳞癌中的表达,以期为口腔鳞癌的诊断和预防提供依据。方法分别采用实时定量聚合酶链反应、免疫印迹方法和免疫组化方法检测口腔黏膜上皮体外癌变模型、口腔鳞癌细胞株及30例原发口腔鳞癌标本中的CK17 mRNA及蛋白质的表达。结果与永生化HIOEC细胞相比,CK17 mRNA在HB56和OSC细胞株中表达明显升高,CK17蛋白表达水平在所有细胞株中均有不同程度的升高。口腔鳞癌患者癌组织中CK17的mRNA和蛋白表达水平均显著高于癌旁组织(P<0.01)。结论 CK17表达上调可能与口腔鳞癌的发生、发展有关。

Objective To investigate the expression of cytokeratin 17（CK17） in oral squamous cell carcinoma（OSCC） as well as its clinical significance.Methods Detection of the mRNA level and protein expression of CK17 in the in vitro cellular carcinogenesis model of OSCC,some OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction（PCR）,Western blot and immunohistochemistry,respectively. Results Increased CK17 mRNA level was observed in the HB56 and OSC cell lines compared with the HIOEC using real-time PCR technique.Western blot showed increased CK17 protein expression in all the cell lines compared with the HIOEC.Increased CK17 mRNA and immunoreaction levels were also observed in the cancerous tissue specimens from OSCC patients compared with normal adjacent tissues（P0.01）.Conclusion The significantly increased CK17 gene may be associated with the tumorigenesis and development of OSCC.