声动力化学疗法对大鼠颅内胶质瘤的杀伤作用

Killing effect of sonodynamic therapy on rat glioma cells

目的探讨声动力化学疗法(SDT)对大鼠颅内胶质瘤的杀伤效应。方法制作大鼠颅内C6胶质瘤实验模型,将大鼠随机分为声动力治疗(SDT)组、超声(US)组、血啉甲醚(HMME)组、对照组,治疗后行颅内胶质瘤MRI影像学检查及组织光镜、电镜观察,并记录4组大鼠生存期。结果 MRI显示:治疗3 d、7 d后,SDT组肿瘤体积分别为(47.05±1.96)mm3、(13.02±1.15)mm3,较其他3组明显减小(均P<0.01)。光镜显示:SDT组肿瘤组织坏死于治疗后24 h达到高峰。电镜显示:治疗后24 h,SDT组见大量坏死细胞,部分细胞呈凋亡表现;US组少量细胞呈凋亡表现。SDT组生存期为52.25 d,较其他3组明显延长(均P<0.01)。结论 SDT对大鼠颅内C6胶质瘤有明显抑制效应。

Objective To investigate the killing effect of sonodynamic therapy(SDT) on rat brain glioma.Methods The rat brain C6 glioma was established as experimental model.The rats were randomly divided into sonodynamic therapy(SDT) group,ultrasound(US) group,hematoporphyrin monomethyl ether(HMME) group and control group.The rat brain glioma of 4 groups was examined by MRI and the tumor tissue was observed by light microscope and electron microscope after treatment.Moreover,the rat survival time of 4 groups was recorded.Results MRI showed that the tumor volume were 47.05±1.96 mm3 and 13.02±1.15 mm3 3 d and 7 d after treatment in SDT group respectively,which were significantly less than that of the other 3 groups(all P<0.01).Light microscope showed that tumor necrosis reached peak 24 h after treatment in SDT group.Electron microscopy showed the necrosis of a large number of cells and apoptosis of part of cells 24 h after treatment in SDT group.A small number of apoptotic cells were seen in US group.The survival time of SDT group was 52.25 d,which was significantly longer than the other 3 groups(all P<0.01).Conclusions SDT can inhibit intracranial C6 rat glioma significantly.