摘要
目的探讨β-淀粉样肽（Ap）对人SH—SY5Y神经母细胞瘤细胞突触素（syn）、发动蛋白I（DynI）及衔接蛋白180（AP180）表达的影响。方法分别应用0．01、0．1、1、2及5-μmol／LAβ1-42处理SH-SY5Y细胞，对照组细胞不加任何处理。MTT法检测细胞增殖情况，western blotting检测0．5、1μmol／LAμ1-42处理组及对照组细胞Syn、DynI和AP180蛋白表达，RT—PCR检测1μmol／LAβ1-42处理组及对照组细胞Syn、DynI和AP180mRNA表达。结果0．1μmol／LAl3Im处理细胞即可出现细胞增殖率下降，并呈剂量依赖性负性相关关系．与对照组比较差异均有统计学意义（P〈0．05）；0．5μmol／LAB1-42处理细胞可见DynI蛋白表达降低，与对照组比较差异有统计学意义（P〈0．05）；1μmol／LAβ1-42处理细胞可见Syn、DynI蛋白及mRNA表达均降低，与对照组比较差异均有统计学意义（P〈0．05）；但未见AP180蛋白及mRNA表达水平发生改变。结论AB1-42可引起人SH-SY5Y细胞Syn和DynI表达降低，该改变可能与AD发病中学习记忆能力减退有关。
Objective To study the effect of [3-amyloid peptide （Aβ1-42） on expressions of synaptophysin （Syn）, dynamin I （Dyn I） and adaptor protein 180 （AP180） in human neuroblastoma SH-SY5Y cells. Methods Human SH-SY5Y neuroblastoma cells were treated with 0.01, 0.1, 1, 2 and 5 μmol/L Aβ1-42, and control cells were given no treatment. MTT [3-（4,5-dimethylthiazol- 2- yl） - 2, 5- diphenyltetrazolium bromide] reduction of the cells was measured by spectrophotometry. The protein levels of Syn, Dyn I and AP180 in SH-SY5Y cells treated with 0.5 and i μmol/L Aβ1-2 were surveyed by Western blotting. The mRNA levels of Syn, Dyn I and AP180 were detected by Real-time PCR in SH-SY5Y cells treated with 1 μmol/L Aβ1-42. Results SH-SY5Y cells showed obviously decreased reduction rates of MTT after exposure to Aβ1-42（0.1 μmol/L） as compared with the controls （P〈0.05）, and dose-dependent negative correlation was noted in these SH-SY5Y cells. The protein level of Dyn I in cells treated with 0.5 μmol/L Aβ1-42 was significantly decreased as compared with that in controls （P〈0.05）. The protein and mRNA levels of Syn and Dyn I in cells treated with 1 μmol/L Aβ1.42 were obviously decreased as compared with those in controls （P〈0.05）, but the levels of AP180 were not changed. Conclusion Aβ1.42 reduces the levels of Syn and Dyn I in SH-SY5Y cells, which might be a mechanism in connection with cognitive deficit of AD.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2011年第8期774-778,共5页
Chinese Journal of Neuromedicine
基金
基金项目：国家自然科学基金（30870986）
贵阳市科学技术计划项目（[2009]3-012）
贵阳医学院研究生教育创新基地建设专项经费
