意向运动疗法对大鼠脑缺血再灌注损伤后NT-3及GAP-43表达的影响

Effect of willed movement therapy on the expression of neurotrophin 3 and growth-associated protein 43 in rats with cerebral ischemia reperfusion

目的观察意向运动疗法对脑缺血再灌注损伤后缺血区脑组织NT-3及GAP-43蛋白表达的影响,探讨意向运动疗法对神经再生和修复的作用。方法运用线栓法制备大鼠大脑中动脉缺血再灌注损伤模型,随机分为大脑中动脉栓塞模型组(MCAO组)、饲养环境改变组(EM组)、意向运动疗法组(WM组),再灌注后3、7、15 d 3个时间点进行神经功能缺损评分、免疫组化法检测脑缺血区脑组织NT-3、GAP-43蛋白的表达。结果再灌注15 d时WM组神经功能缺损评分显著低于MCAO组及EM组(P<0.05);再灌注7、15 d时WM组的NT-3及GAP-43蛋白表达均显著高于MCAO组和EM组(P<0.05);EM组与MCAO组比较:NT-3及GAP-43蛋白的表达无明显差异(P>0.05);NT-3与GAP-43蛋白的表达变化呈正相关。结论意向运动疗法可促进脑缺血再灌注损伤大鼠缺血区脑组织NT-3、GAP-43蛋白的表达,可能与缺血损伤神经元的再生和修复有关。

Objective To observe the effect of willed movement therapy on the expression of neurotrophin 3（NT-3） and growth associated protein 43（GAP-43） in rats with cerebral ischemia-reperfusion（IR） and investigate the neuroprotective mechanism of willed movement therapy in nerve regeneration and repair.Methods Cerebral IR model was established by middle cerebral artery occlusion（MCAO） in SD rats.The rats were randomly divided into MCAO group,environment modification group（EM group） and willed movement therapy group（WM group）.The rats were evaluated for neurological deficits and decapitated on days 3,7 and 15 after the reperfusion to examine the expressions of NT-3 and GAP-43 in the ischemic brain tissues by immunohistochemistry.Results Compared with MCAO and EM groups,the rats in WM group showed significantly lowered grade of neurological deficits（P0.05） at 15 days and significantly increased the expressions of NT-3 and GAP-43（P0.05） at 7 and 15 days after the reperfusion.No significant difference was found in the expression of NT-3 and GAP-43 between MCAO and EM groups（P0.05）.The expression of NT-3 was positively correlated to that of GAP-43 in the ischemic tissues.Conclusion Willed movement therapy increases the expression of NT-3 and GAP-43 in the ischemic brain area in rats with cerebral ischemia-reperfusion,which is probably related to nerve regeneration and repair.