金属硫蛋白对罗哌卡因灌注下离体兔心肌细胞凋亡和心肌细胞能供的影响

Effect of metallothionein on myocyte apoptosis and energy supply of isolated rabbit heart muscle during perfusion with ropivacaine

目的探讨金属硫蛋白对罗哌卡因灌注下的离体兔心肌细胞凋亡和心肌细胞能供的影响。方法成年雄性新西兰大组肌灌脏胞Ⅲ白能凋Ⅰ显兔注行量亡和著液6L0a供率组高中n只g给及Ⅱ于输e,n随障心;组注d心o机碍肌Ⅱ罗f肌f分。三灌。哌细为磷注卡结胞腹酸及因论凋腔腺灌组金亡(注苷注属率组(射液A硫组Ⅲ蒸中T)蛋ⅡP馏)输。白显、水注在二能著,罗灌磷2显高4哌注酸著于h卡液腺后抑组因中苷取制Ⅰ(组输离罗和A(注体D哌组组P罗心)卡ⅢⅡ、哌脏)因,环组;卡行引酸腹Ⅲ因L起腺腔显a1的n苷注0著g(心em射n高Aid肌n3M于o.后细6fPf组%)灌,胞含3ⅠZ注组凋量n;S组动心亡。O(物肌的4组,结2取发4AⅠ果Th心生)P后心;室,含腹取同肌肌量腔时离金分组注显体属别Ⅰ射著心硫测显蒸减脏蛋量著馏轻行白金高水罗含L属于a,哌量2n硫组4g卡组eh蛋Ⅱn后因Ⅲd白和o取引显f含组f灌离起著量Ⅲ注体的高,。

Objective To assess the effects of metallothionein on myocyte apoptosis and energy supply of isolated rabbit heart muscle during perfusion with ropivacaine.Methods Sixty New Zealand white male rabbits were randomized into 3 equal groups.In group I,the rabbits received a intreaperitioneal injection of distilled water 24 h before isolation of the heart with perfusion by Langendoff model;in group II,distilled water was injected intreaperitioneally,and 24 h later the heart was isolated and perfused with Langendoff model and ropivacaine;in group III,3.6% ZnSO4 was injected intreaperitioneally and the isolated heart was perfused with Langendoff model and ropivacaine.The myocardial metallothionein content,myocyte apoptosis,and myocardial ATP,ADP and AMP content were detected.Results The myocardial metallothionein content was significantly higher in group III than in the other two groups;the percent of myocyte apoptosis was the highest in group II,and was significantly higher in group III than in group I.The myocardial content of ATP was the highest in group I,and was significantly higher in group III than in group II.Conclusion Metallothionein can significantly inhibit myocyte apoptosis and alleviate energy supply disorder induced by ropivacaine.