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H~＋-ATP酶定位的电镜细胞化学观察 被引量：1

Ultracytochemical observation of the intracellular localization of H^+-adenosine triphos-phatase
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摘要 目的用电子显微镜细胞化学方法观察H+-ATP酶在细胞器中的位置。H+-ATP酶不仅定位在溶酶体膜,而且定位在Wistar大鼠近曲小管上皮细胞的细胞膜、核膜上。上述结果表明用电镜细胞化学方法以形态学表现形式证实H+-ATP酶定位在细胞内膜相结构的学说。 Objective To observe the ultracytochemical localization of H＋-adenosine triphosphatase（H＋-ATPase） in the cell organelles.Methods The localization of H＋-ATPase in the cell organelles was observed in the hepatocytes and renal cells of Wistar rats using routine ultracytochemical methods.Results H＋-ATPase activities were observed on the lysosomal membrane and nuclear envelope of the hepatocytes and proximal tubule epithelial cells of the nephron in Wistar rats.Conclusion This finding supports the hypothesis that H＋-ATPase（V-ATPase） is present on the plasma membrane and in the endomembrane system.
作者 罗深秋 柯志勇 路艳蒙
机构地区 南方医科大学细胞生物学教研室 南方医科大学细胞生物学教研室电镜室
出处 《南方医科大学学报》 CAS CSCD 北大核心 2011年第8期1431-1433,共3页 Journal of Southern Medical University
基金 广东省自然科学基金(101510515D1000040)
关键词 H+-ATP酶 电子显微镜细胞化学 H＋-adenosine triphosphatase ultracytochemical localization
分类号 Q55 [生物学—生物化学]
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参考文献13

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南方医科大学学报
2011年 第8期

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