肝癌缺失基因1和磷酸化粘着斑激酶蛋白在乳腺癌组织中的表达及意义

Expression of deleted in liver cancer 1 and phosphorelated focal adhesion kinase in breast cancer

目的研究肝癌缺失基因1(DLC1)和磷酸化粘着斑激酶(FAK)在乳腺癌组织中的表达及其与临床病理指标的关系,加深对乳腺癌癌变和转移分子机制的理解。方法采用免疫组化ABC法检测61例乳腺癌和30例乳腺良性纤维瘤及其周围正常组织中DLC1和磷酸化FAK蛋白的表达情况;并结合临床病理资料分析二者在乳腺癌中表达的意义,采用SPSS10.0统计学软件分析数据。结果 DLC1在乳腺癌、良性组织和正常组织中的表达率分别为34.43%,80.00%和76.67%(P<0.001),磷酸化FAK在3组中的表达率为77.05%,33.33%和26.67%(P<0.001)。并且DLC1和磷酸化FAK蛋白在乳腺癌组织中的表达呈明显负相关(Kappa值=-0.4591);DLC1和FAK均与乳腺癌的发生、分期、PR和淋巴结转移关系密切(P<0.05),而与乳腺癌的年龄、家族史、分型、雌激素(ER)和CerbB-2无明显相关性(P>0.05)。结论 DLC1低或无表达和磷酸化FAK高表达与乳腺癌的发生发展有关,DLC1和磷酸化FAK的表达与孕激素受体表达有关,有可能成为乳腺癌早诊和预后判断的候选标志物。

Objective To analyze the expression of deleted in liver cancer 1（DLC1） and phosphorelated focal adhesion kinase（p-FAK） in breast cancer tissue to further understand the molecular mechanisms of the carcinogenesis and metastasis of breast cancer.Methods Immunohistochemistry was employed to determine the protein level of DLC1 and p-FAK in 61 breast cancer,30 benign breast disease and the adjacent normal breast tissues.Results The positivity rates of DLC1 differed significantly between breast cancer,benign and normal tissues（34.43%,80.00% and 76.67%,respectively,P0.001）.The positivity rates of p-FAK in the 3 tissues were 77.05%,33.33% and 26.67%,also showing significant differences（P0.001）.The aberrant expression of DLC1 showed an inverse correlation to p-FAK（κ=-0.4591）.Both DLC1 and p-FAK were closely correlated to the carcinogenesis,clinical stage,PR and lymphatic metastasis of breast cancer（P0.05）,but not to the patients＇ age,pathological subtype,familial history,ER or CerbB-2（P0.05）.Conclusions The abnormal expression of DLC1 and p-FAK might participate in the carcinogenesis,progression,and metastasis of breast cancer.The role of DLC1 and p-FAK might be related to the regulation of progestone.DLC1 and p-FAK may serve as candidate markers for early diagnosis,prognostic evaluation and target treatment of breast cancer.