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肝癌缺失基因1和磷酸化粘着斑激酶蛋白在乳腺癌组织中的表达及意义 被引量:7

Expression of deleted in liver cancer 1 and phosphorelated focal adhesion kinase in breast cancer
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摘要 目的研究肝癌缺失基因1(DLC1)和磷酸化粘着斑激酶(FAK)在乳腺癌组织中的表达及其与临床病理指标的关系,加深对乳腺癌癌变和转移分子机制的理解。方法采用免疫组化ABC法检测61例乳腺癌和30例乳腺良性纤维瘤及其周围正常组织中DLC1和磷酸化FAK蛋白的表达情况;并结合临床病理资料分析二者在乳腺癌中表达的意义,采用SPSS10.0统计学软件分析数据。结果 DLC1在乳腺癌、良性组织和正常组织中的表达率分别为34.43%,80.00%和76.67%(P<0.001),磷酸化FAK在3组中的表达率为77.05%,33.33%和26.67%(P<0.001)。并且DLC1和磷酸化FAK蛋白在乳腺癌组织中的表达呈明显负相关(Kappa值=-0.4591);DLC1和FAK均与乳腺癌的发生、分期、PR和淋巴结转移关系密切(P<0.05),而与乳腺癌的年龄、家族史、分型、雌激素(ER)和CerbB-2无明显相关性(P>0.05)。结论 DLC1低或无表达和磷酸化FAK高表达与乳腺癌的发生发展有关,DLC1和磷酸化FAK的表达与孕激素受体表达有关,有可能成为乳腺癌早诊和预后判断的候选标志物。 Objective To analyze the expression of deleted in liver cancer 1(DLC1) and phosphorelated focal adhesion kinase(p-FAK) in breast cancer tissue to further understand the molecular mechanisms of the carcinogenesis and metastasis of breast cancer.Methods Immunohistochemistry was employed to determine the protein level of DLC1 and p-FAK in 61 breast cancer,30 benign breast disease and the adjacent normal breast tissues.Results The positivity rates of DLC1 differed significantly between breast cancer,benign and normal tissues(34.43%,80.00% and 76.67%,respectively,P0.001).The positivity rates of p-FAK in the 3 tissues were 77.05%,33.33% and 26.67%,also showing significant differences(P0.001).The aberrant expression of DLC1 showed an inverse correlation to p-FAK(κ=-0.4591).Both DLC1 and p-FAK were closely correlated to the carcinogenesis,clinical stage,PR and lymphatic metastasis of breast cancer(P0.05),but not to the patients' age,pathological subtype,familial history,ER or CerbB-2(P0.05).Conclusions The abnormal expression of DLC1 and p-FAK might participate in the carcinogenesis,progression,and metastasis of breast cancer.The role of DLC1 and p-FAK might be related to the regulation of progestone.DLC1 and p-FAK may serve as candidate markers for early diagnosis,prognostic evaluation and target treatment of breast cancer.
出处 《南方医科大学学报》 CAS CSCD 北大核心 2011年第8期1448-1451,共4页 Journal of Southern Medical University
关键词 乳腺癌 抑癌基因 肝癌缺失基因1 磷酸化粘着斑激酶 免疫组化 breast cancer tumor suppressor genes deleted in liver cancer 1 phosphorelated focal adhesion kinase immunohistochemistry
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同被引文献37

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