皮肤血管瘤患儿尿液中碱性成纤维细胞生长因子的检测

Determination of basic fibroblast growth factor （bFGF） level in urine from infants and young children with cutaneous hemangioma

目的 检测尿液中碱性成纤维细胞生长因子（bFGF）水平在血管瘤病程中的变化,为临床监测血管瘤的发生发展提供客观依据.方法 采用酶联免疫吸附法（ELISA）检测27例增生期皮肤血管瘤患儿浅层X线治疗前后尿液中bFGF水平的变化,同时选择30例健康婴幼儿尿液作为对照.结果 增生期血管瘤患儿治疗前尿液中bFGF水平高于治疗后3个月和对照组,差异有统计学意义（t=0.206,P＜0.05）,治疗后3个月与对照组之间bFGF水平的差异无统计学意义（t=0.505,P＞0.05）.结论 ELISA检测尿液中bFGF方法简便、可靠,可为临床监测血管瘤发生发展提供客观指标.

Objective To determine the changes of urine bFGF level in the progression of cutaneous hemangioma,and to provide an objective index for the monitoring of hemangioma occurrence and development.Methods Enzyme-linked immunosorbent assay （ELISA） was used to determine the bFGF level in urine samples from 27 infants and young children with proliferative cutaneous hemangioma before and after treatment with low-dose superficial X-ray,and from 30 normal control infants and young children.Results The urine bFGF level was significantly higher in the patients before treatment than in those 3 months after the treatment and normal controls（t = 3.793,0.206,both P 〈 0.05）,while no significant difference was observed in the urine bFGF level between the treated patients and controls （t = 0.505,P 〉 0.05）.Conclusions Measurement of urine bFGF level by ELISA is a convenient and reliable method ,which may provide an objective index for the monitoring of hemangioma occurrence and development.